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Anti-Phospho-FAK (Tyr397) Polyclonal Antibody
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Catalog No. TMAB-01411
Alias PTK 2, Protein-tyrosine kinase 2, Protein Tyrosine Kinase Cytoplasmic, Protein tyrosine kinase 2, Protein phosphatase 1 regulatory subunit 71, PPP1R71, pp125FAK, p-FAK (Y397), p-FAK (Tyr397), p125FAK, FRNK, Focal adhesion kinase-related nonkinase, Focal adhesion kinase 1, FAK1, FAK related non kinase polypeptide, FAK 1, FAK (p-Y397), FAK (p-Tyr397), FADK 1, FADK
Anti-Phospho-FAK (Tyr397) Polyclonal Antibody is a Rabbit antibody targeting Phospho-FAK (Tyr397). Anti-Phospho-FAK (Tyr397) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Anti-Phospho-FAK (Tyr397) Polyclonal Antibody
Anti-Phospho-FAK (Tyr397) Polyclonal Antibody
😃Good
Catalog No. TMAB-01411Alias PTK 2, Protein-tyrosine kinase 2, Protein Tyrosine Kinase Cytoplasmic, Protein tyrosine kinase 2, Protein phosphatase 1 regulatory subunit 71, PPP1R71, pp125FAK, p-FAK (Y397), p-FAK (Tyr397), p125FAK, FRNK, Focal adhesion kinase-related nonkinase, Focal adhesion kinase 1, FAK1, FAK related non kinase polypeptide, FAK 1, FAK (p-Y397), FAK (p-Tyr397), FADK 1, FADK
Anti-Phospho-FAK (Tyr397) Polyclonal Antibody is a Rabbit antibody targeting Phospho-FAK (Tyr397). Anti-Phospho-FAK (Tyr397) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
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Antigen Details
Chemical Properties
| Description | Anti-Phospho-FAK (Tyr397) Polyclonal Antibody is a Rabbit antibody targeting Phospho-FAK (Tyr397). Anti-Phospho-FAK (Tyr397) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF. |
| Alias | PTK 2, Protein-tyrosine kinase 2, Protein Tyrosine Kinase Cytoplasmic, Protein tyrosine kinase 2, Protein phosphatase 1 regulatory subunit 71, PPP1R71, pp125FAK, p-FAK (Y397), p-FAK (Tyr397), p125FAK, FRNK, Focal adhesion kinase-related nonkinase, Focal adhesion kinase 1, FAK1, FAK related non kinase polypeptide, FAK 1, FAK (p-Y397), FAK (p-Tyr397), FADK 1, FADK |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Chicken,Dog,Cow,Horse,Rabbit) |
| Verified Activity | 1. Sample: Thymus (Mouse) Lysate at 40 μg Primary: Anti-p-FAK (Tyr397) (TMAB-01411) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 116 kDa Observed band size: 116 kDa 2. Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (P-FAK (Tyr397)) Polyclonal Antibody, Unconjugated (TMAB-01411) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 3. Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 μg Lane 2: Testis (Mouse) Lysate at 40 μg Lane 3: NIH/3T3 (Mouse) Cell Lysate at 30 μg Lane 4: Cerebrum (Rat) Lysate at 40 μg Lane 5: Adrenal gland (Rat) Lysate at 40 μg Lane 6: Testis (Rat) Lysate at 40 μg Lane 7: 293T (Human) Cell Lysate at 30 μg Lane 8: A431 (Human) Cell Lysate at 30 μg Lane 9: Huvec (Human) Cell Lysate at 30 μg Lane 10: MDA-MB-231 (Human) Cell Lysate at 30 μg Lane 11: A549 (Human) Cell Lysate at 30 μg Primary: Anti-Phospho-FAK (Tyr397) (TMAB-01411) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 125 kDa Observed band size: 125 kDa 4. Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-FAK (Tyr397)) Polyclonal Antibody, Unconjugated (TMAB-01411) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 5. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (Phospho-FAK (Tyr397)) polyclonal Antibody, Unconjugated (TMAB-01411) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:50; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, centrosome. Nucleus. Note=Constituent of focal adhesions. Detected at microtubules. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity. [SUBCELLULAR LOCATION] Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=Constituent of focal adhesions. |
| Immunogen | KLH cunjugated Synthesised phosphopeptide: human FAK around the phosphorylation site of Tyr397 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | FAK family,Integrins,Focal Adhesions,FAK / PYK |
| Molecular Weight | Theoretical: 116 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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