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Anti-Phospho-EGF Receptor (Thr669) Antibody (8Z529) is a Rabbit antibody targeting Phospho-EGF Receptor (Thr669). Anti-Phospho-EGF Receptor (Thr669) Antibody (8Z529) can be used in WB.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
20 μL | $80 | 7-10 days | |
100 μL | $235 | 7-10 days |
Description | Antibody Type: Rabbit Monoclonal Application: WB Reactivity: Human |
Alias | PIG61, Phospho-EGF Receptor (T669), p-EGF Receptor (Thr669), p-EGF Receptor (T669), NISBD2, mENA, HER-1, HER1, ERRP, ERBB-1, ERBB1, ERBB, EGF Receptor (pThr669), EGF Receptor (pT669) |
Ig Type | Rabbit IgG |
Clone | 8Z529 |
Reactivity | Human;
Predicted to React with:Species predicted to react based on 100% sequence homology: Mouse, Cynomolgus |
Verified Activity | 1. Western blot analysis of extracts from serum-starved Hela, untreated(line A) or treated with EGF (100ng/mL, 10min; +)(line B), using Phospho-EGF Receptor (Thr669) rabbit monoclonal Antibody at 1:2000 dilution (upper) or Anti-EGF Receptor Antibody, Rabbit Polyclonal at 1:1000 dilution (lower). 2. Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with EGF (100ng/mL, 10min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-EGF Receptor (Thr669) rabbit monoclonal Antibody at 1:2000 dilution. (Validation Experiment) |
Application | WB |
Recommended Dose | WB: 1:2000-1:20000 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | This antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to the residues around (Thr669) of Human EGF Receptor. |
Purification | Protein A |
Appearance | Liquid |
Formulation | 10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL BSA, 50% glycerol, pH 7.5 |
Research Background | As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
Conjucates | Unconjugated |
Immunogen | A synthetic peptide: residues around (Thr669) of Human EGF Receptor |
Antigen Species | Human |
Biology Area | Cancer Drug Targets, Receptor Tyrosine Kinases (RTKs) |
Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
Transport | Shipping with blue ice. |
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