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Anti-Phospho-BRAF (Ser365) Polyclonal Antibody
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Catalog No. TMAB-01396
Alias v-Raf murine sarcoma viral oncogene homolog B1, Serine/threonine-protein kinase B-raf, RAFB1, Proto-oncogene B-Raf, p-BRAF (Ser365), p-BRAF (S365), p94, EC 2.7.11.1, BRAF1, BRAF (p-Ser365), BRAF (p-S365)
Anti-Phospho-BRAF (Ser365) Polyclonal Antibody is a Rabbit antibody targeting Phospho-BRAF (Ser365). Anti-Phospho-BRAF (Ser365) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Anti-Phospho-BRAF (Ser365) Polyclonal Antibody
Anti-Phospho-BRAF (Ser365) Polyclonal Antibody
😃Good
Catalog No. TMAB-01396Alias v-Raf murine sarcoma viral oncogene homolog B1, Serine/threonine-protein kinase B-raf, RAFB1, Proto-oncogene B-Raf, p-BRAF (Ser365), p-BRAF (S365), p94, EC 2.7.11.1, BRAF1, BRAF (p-Ser365), BRAF (p-S365)
Anti-Phospho-BRAF (Ser365) Polyclonal Antibody is a Rabbit antibody targeting Phospho-BRAF (Ser365). Anti-Phospho-BRAF (Ser365) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Antigen Details
Chemical Properties
| Description | Anti-Phospho-BRAF (Ser365) Polyclonal Antibody is a Rabbit antibody targeting Phospho-BRAF (Ser365). Anti-Phospho-BRAF (Ser365) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF. |
| Alias | v-Raf murine sarcoma viral oncogene homolog B1, Serine/threonine-protein kinase B-raf, RAFB1, Proto-oncogene B-Raf, p-BRAF (Ser365), p-BRAF (S365), p94, EC 2.7.11.1, BRAF1, BRAF (p-Ser365), BRAF (p-S365) |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-B-Raf (Ser365)) Polyclonal Antibody, Unconjugated (TMAB-01396) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-B-Raf (Ser365)) Polyclonal Antibody, Unconjugated (TMAB-01396) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (rat placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-B-Raf (Ser365)) Polyclonal Antibody, Unconjugated (TMAB-01396) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-B-Raf (Ser365)) Polyclonal Antibody, Unconjugated (TMAB-01396) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-B-Raf (Ser365)) Polyclonal Antibody, Unconjugated (TMAB-01396) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. Cytoplasm. Cell membrane. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome. [provided by RefSeq, Aug 2017] |
| Immunogen | KLH conjugated Synthesised phosphopeptide: human BRAF around the phosphorylation site of Ser365 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Signal transducers,Kinases,Cell differentiation,Other Kinases,Tyrosine Kinases |
| Molecular Weight | Theoretical: 84 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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