Anti-Phospho-BRAF (Ser365) Polyclonal Antibody is a Rabbit antibody targeting Phospho-BRAF (Ser365). Anti-Phospho-BRAF (Ser365) Polyclonal Antibody can be used in IF, IHC-Fr, IHC-P.

IgG

Mouse,Rat (predicted:Human,Chicken,Dog,Pig,Cow,Horse,Rabbit)

1. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-B-Raf (Ser365)) Polyclonal Antibody, Unconjugated (TMAB-01396) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-B-Raf (Ser365)) Polyclonal Antibody, Unconjugated (TMAB-01396) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (rat placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-B-Raf (Ser365)) Polyclonal Antibody, Unconjugated (TMAB-01396) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-B-Raf (Ser365)) Polyclonal Antibody, Unconjugated (TMAB-01396) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-B-Raf (Ser365)) Polyclonal Antibody, Unconjugated (TMAB-01396) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated Synthesised phosphopeptide: human BRAF around the phosphorylation site of Ser365

Human

BRAF

Serine/threonine-protein kinase B-raf

Signal transducers,Kinases,Cell differentiation,Other Kinases,Tyrosine Kinases

Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.