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Anti-PEDF Polyclonal Antibody

😃Good
Catalog No. TMAB-01365
Alias serpin peptidase inhibitor, clade F,member 1, PIG35, PEDF, OI6, OI12, EPC-1

Anti-PEDF Polyclonal Antibody is a Rabbit antibody targeting PEDF. Anti-PEDF Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-PEDF Polyclonal Antibody

Anti-PEDF Polyclonal Antibody

😃Good
Catalog No. TMAB-01365Alias serpin peptidase inhibitor, clade F,member 1, PIG35, PEDF, OI6, OI12, EPC-1
Anti-PEDF Polyclonal Antibody is a Rabbit antibody targeting PEDF. Anti-PEDF Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2217-10 days
100 μL$3727-10 days
200 μL$5287-10 days
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Product Introduction

Bioactivity
Description
Anti-PEDF Polyclonal Antibody is a Rabbit antibody targeting PEDF. Anti-PEDF Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Aliasserpin peptidase inhibitor, clade F,member 1, PIG35, PEDF, OI6, OI12, EPC-1
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Pig,Cow,Sheep)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (Human glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PEDF) Polyclonal Antibody, Unconjugated (TMAB-01365) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (Mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PEDF) Polyclonal Antibody, Unconjugated (TMAB-01365) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
3. Sample: Liver (Mouse) Lysate at 40 μg
Primary: Anti-PEDF (TMAB-01365) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kDa
Observed band size: 46 kDa
4. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PEDF) Polyclonal Antibody, Unconjugated (TMAB-01365) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationSecreted. Melanosome. Enriched in stage I melanosomes.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundPigment epithelium derived factor, originally identified in conditioned medium of cultured human fetal retinal pigment epithelial (RPE) cells, is a neurotrophic protein that induces extensive neuronal differentiation in human Y79 retinoblastoma cells, a neoplastic counterpart of normal retinoblasts. It has been suggested that PEDF is synthesized by RPE cells and secreted into the retina interphotoreceptor matrix where it may influence development/differentiation of the neural retina. PEDF is a potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. The PEDF gene is a member of the serpin gene family. Serpins are a group of serine protease inhibitors, some of which have also been reported to exhibit neurotrophic activity.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human PEDF
Antigen Species
Human
Gene ID
Uniprot ID
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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