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Anti-PEDF Polyclonal Antibody
😃Good
Catalog No. TMAB-01365
Alias serpin peptidase inhibitor, clade F，member 1, PIG35, PEDF, OI6, OI12, EPC-1
Anti-PEDF Polyclonal Antibody is a Rabbit antibody targeting PEDF. Anti-PEDF Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-PEDF Polyclonal Antibody
Anti-PEDF Polyclonal Antibody
😃Good
Catalog No. TMAB-01365Alias serpin peptidase inhibitor, clade F，member 1, PIG35, PEDF, OI6, OI12, EPC-1
Anti-PEDF Polyclonal Antibody is a Rabbit antibody targeting PEDF. Anti-PEDF Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-PEDF Polyclonal Antibody is a Rabbit antibody targeting PEDF. Anti-PEDF Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | serpin peptidase inhibitor, clade F，member 1, PIG35, PEDF, OI6, OI12, EPC-1 |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Pig,Cow,Sheep) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Human glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PEDF) Polyclonal Antibody, Unconjugated (TMAB-01365) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (Mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PEDF) Polyclonal Antibody, Unconjugated (TMAB-01365) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 3. Sample: Liver (Mouse) Lysate at 40 μg Primary: Anti-PEDF (TMAB-01365) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kDa Observed band size: 46 kDa 4. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PEDF) Polyclonal Antibody, Unconjugated (TMAB-01365) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted. Melanosome. Enriched in stage I melanosomes. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Pigment epithelium derived factor, originally identified in conditioned medium of cultured human fetal retinal pigment epithelial (RPE) cells, is a neurotrophic protein that induces extensive neuronal differentiation in human Y79 retinoblastoma cells, a neoplastic counterpart of normal retinoblasts. It has been suggested that PEDF is synthesized by RPE cells and secreted into the retina interphotoreceptor matrix where it may influence development/differentiation of the neural retina. PEDF is a potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. The PEDF gene is a member of the serpin gene family. Serpins are a group of serine protease inhibitors, some of which have also been reported to exhibit neurotrophic activity. |
| Immunogen | KLH conjugated synthetic peptide: human PEDF |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID |
| Molecular Weight | Theoretical: 46 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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