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Anti-NRP1 Antibody (4D9)
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Catalog No. TMAC-02788
Alias VEGF165R, NRP, NP1, Neuropilin-1, neuropilin 1, CD304, BDCA4
Anti-NRP1 Antibody (4D9) is a Rabbit antibody targeting NRP1. Anti-NRP1 Antibody (4D9) can be used in WB,ICC/IF,IHC,IP,FCM.
Anti-NRP1 Antibody (4D9)
Anti-NRP1 Antibody (4D9)
😃Good
Catalog No. TMAC-02788Alias VEGF165R, NRP, NP1, Neuropilin-1, neuropilin 1, CD304, BDCA4
Anti-NRP1 Antibody (4D9) is a Rabbit antibody targeting NRP1. Anti-NRP1 Antibody (4D9) can be used in WB,ICC/IF,IHC,IP,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $298 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
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Chemical Properties
| Description | Anti-NRP1 Antibody (4D9) is a Rabbit antibody targeting NRP1. Anti-NRP1 Antibody (4D9) can be used in WB,ICC/IF,IHC,IP,FCM. |
| Synonyms | VEGF165R, NRP, NP1, Neuropilin-1, neuropilin 1, CD304, BDCA4 |
| Ig Type | IgG |
| Clone | 4D9 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of Neuropilin-1 on different lysates using anti-Neuropilin-1 antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse heart, Lane 2: Mouse liver, Lane 3: Mouse kidney, Lane 4: Human liver. 2. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Neuropilin-1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Neuropilin-1 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Neuropilin-1 antibody. Counter stained with hematoxylin. 5. ICC staining Neuropilin-1 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining Neuropilin-1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining Neuropilin-1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. Flow cytometric analysis of Hela cells with Neuropilin-1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Neuropilin is a type I transmembrane receptor that has been implicated in aspects of axon growth and guidance and has been shown to act as a high affinity receptor for class III semaphorins and vascular endothelial growth factor (VEGF). A closely related protein, neuropilin-2, shares a common domain structure and signifcant homology with neuropilin and also acts as a receptor for the class III semaphorins and VEGF. Both neuropilins are involved in regulating many physiological pathways including axonal guidance and angiogenesis, however they exhibit differential expression in the adult vasculature. Neuropilin-2 is polysialylated and expressed on the surface of dendritic cells. It is also expressed by venous and lymphatic endothelium. Neuropilin is expressed predominantly by arterial endothelium. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 120 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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