Antibody Type: Rabbit Polyclonal

Application: WB,IHC-P,IHC-Fr,IF,FCM

Reactivity: Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Sheep)

IgG

Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Sheep)

1. Blank control: A549. Primary Antibody (green line): Rabbit Anti-Glucocorticoid Receptor antibody (TMAB-01267)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
2. Sample:
Lane 1: Raw264.7 (Mouse) Cell Lysate at 30 μg
Lane 2: Stomach (Rat) Lysate at 40 μg
Lane 3: Stomach (Mouse) Lysate at 40 μg
Lane 4: Cerebrum (Rat) Lysate at 40 μg
Lane 5: Cerebrum (Mouse) Lysate at 40 μg
Lane 6: Testis (Rat) Lysate at 40 μg
Lane 7: Testis (Mouse) Lysate at 40 μg
Lane 8: Liver (Rat) Lysate at 40 μg
Lane 9: Liver (Mouse) Lysate at 40 μg
Primary: Anti-Glucocorticoid Receptor (TMAB-01267) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 90 kDa
Observed band size: 87 kDa
3. Blank control: Mouse spleen.
Primary Antibody (green line): Rabbit Anti-Glucocorticoid Receptor beta antibody (TMAB-01267)
Dilution: 3 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 3 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
4. Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (GCR) Polyclonal Antibody, Unconjugated (TMAB-01267) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

WB,IHC-P,IHC-Fr,IF,FCM

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/Test

Polyclonal

KLH conjugated synthetic peptide: human Glucocorticoid Receptor beta

Human

2908

Nuclear hormone receptors,Corticoid,Corticoid