Anti-NFKB1 Polyclonal Antibody is a Rabbit antibody targeting NFKB1. Anti-NFKB1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow)

1. Sample: Lung (Mouse) lysate at 30 μg;
Primary: Anti-NFkB p105/p50 (TMAB-01233) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit Igg (secondary antibody) at 1: 5000 dilution;
Predicted band size: 48/105 kDa
Observed band size: 110 kDa
2. Sample: Brain (Mouse) lysate at 30 μg;
Primary: Anti-NFkB p105/p50 (TMAB-01233) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit Igg (secondary antibody) at 1: 5000 dilution;
Predicted band size: 48/105 kDa
Observed band size: 50 kD
3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NFkB p50) Polyclonal Antibody, Unconjugated (TMAB-01233) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NFkB p105) Polyclonal Antibody, Unconjugated (TMAB-01233) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (rat bladder); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NFkB p105) Polyclonal Antibody, Unconjugated (TMAB-01233) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NFkB p105) Polyclonal Antibody, Unconjugated (TMAB-01233) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
7. Sample:
Lane 1: Human Raji cell lysates
Lane 2: Human MCF-7 cell lysates
Lane 3: Human Jurkat cell lysates
Primary: Anti-NFKB1 (TMAB-01233) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48/105 kDa
Observed band size: 135 kDa

WB,IHC-P,IHC-Fr,IF

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Nuclear factor NF-kappa-B p50 subunit

Human

p50,NFkB pathway,p50,NFkB pathway,Transcription Factors,Macrophage / Inflamm.,NFkB Pathway