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Anti-Mucin-1/MUC1 Polyclonal Antibody

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Catalog No. TMAB-01184
Alias PUM, PEMT, PEM, mucin 1, cell surface associated, Mucin 1, MUC1/ZD, MUC-1/X, MUC-1/SEC, MUC-1, MCKD1, MCKD, MCD, MAM6, KL-6, H23AG, EMA, CD227, CA15-3, ADMCKD1, ADMCKD

Anti-Mucin-1/MUC1 Polyclonal Antibody is a Rabbit antibody targeting Mucin-1/MUC1. Anti-Mucin-1/MUC1 Polyclonal Antibody can be used in ICC/IF,IF,IHC-Fr,IHC-P.

Anti-Mucin-1/MUC1 Polyclonal Antibody

Anti-Mucin-1/MUC1 Polyclonal Antibody

😃Good
Catalog No. TMAB-01184Alias PUM, PEMT, PEM, mucin 1, cell surface associated, Mucin 1, MUC1/ZD, MUC-1/X, MUC-1/SEC, MUC-1, MCKD1, MCKD, MCD, MAM6, KL-6, H23AG, EMA, CD227, CA15-3, ADMCKD1, ADMCKD
Anti-Mucin-1/MUC1 Polyclonal Antibody is a Rabbit antibody targeting Mucin-1/MUC1. Anti-Mucin-1/MUC1 Polyclonal Antibody can be used in ICC/IF,IF,IHC-Fr,IHC-P.
Pack SizePriceAvailabilityQuantity
50 μL$2207-10 days
100 μL$3737-10 days
200 μL$5287-10 days
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Product Introduction

Bioactivity
Description
Anti-Mucin-1/MUC1 Polyclonal Antibody is a Rabbit antibody targeting Mucin-1/MUC1. Anti-Mucin-1/MUC1 Polyclonal Antibody can be used in ICC/IF,IF,IHC-Fr,IHC-P.
SynonymsPUM, PEMT, PEM, mucin 1, cell surface associated, Mucin 1, MUC1/ZD, MUC-1/X, MUC-1/SEC, MUC-1, MCKD1, MCKD, MCD, MAM6, KL-6, H23AG, EMA, CD227, CA15-3, ADMCKD1, ADMCKD
Ig Type
IgG2b
Reactivity
Human,Mouse,Rat
Verified Activity
1. MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (MUC1) polyclonal Antibody, Unconjugated (TMAB-01184) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
2. Paraformaldehyde-fixed, paraffin embedded (mouse lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MUC1) Polyclonal Antibody, Unconjugated (TMAB-01184) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MUC1) Polyclonal Antibody, Unconjugated (TMAB-01184) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MUC1) Polyclonal Antibody, Unconjugated (TMAB-01184) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MUC1) Polyclonal Antibody, Unconjugated (TMAB-01184) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded (mouse lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MUC1) Polyclonal Antibody, Unconjugated (TMAB-01184) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MUC1) Polyclonal Antibody, Unconjugated (TMAB-01184) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
8. Tissue/cell: MCF-7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (MUC1) Polyclonal Antibody, Unconjugated (TMAB-01184) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (5 μg/ml, blue) was used to stain the cell nucleus.
Application
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationSecreted; Cell membrane. Cytoplasm. Nucleus. On EGF and PDGFRB stimulation, transported to the nucleus through interaction with CTNNB1, a process which is stimulated by phosphorylation. On HRG stimulation, colocalizes with JUP/gamma-catenin at the nucleus and Apical cell membrane. Exclusively located in the apical domain of the plasma membrane of highly polarized epithelial cells. After endocytosis, internalized and recycled to the cell membrane. Located to microvilli and to the tips of long filopodial protusions.
Tissue SpecificityExpressed on the apical surface of epithelial cells, especially of airway passages, breast and uterus. Also expressed in activated and unactivated T-cells. Overexpressed in epithelial tumors, such as breast or ovarian cancer and also in non-epithelial tum
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1 mg/mL
Research BackgroundMUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and overexpressed abundantly in >90% breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four cebB receptors and localize with erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human MUC1
Antigen Species
Human
Gene Name
MUC1
Gene ID
Protein Name
Mucin-1
Uniprot ID
Biology Area
MUC,Tumor-associated antigens,MUC,Tumor Associated
Function
The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.
The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.
Chemical Properties
Molecular WeightTheoretical: 20/138 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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