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Anti-MMP-9 Antibody (5C993) is a Rabbit antibody targeting MMP-9. Anti-MMP-9 Antibody (5C993) can be used in WB,ICC/IF,IHC,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $296 | 7-10 days | |
100 μL | $497 | 7-10 days |
Description | Anti-MMP-9 Antibody (5C993) is a Rabbit antibody targeting MMP-9. Anti-MMP-9 Antibody (5C993) can be used in WB,ICC/IF,IHC,FCM. |
Synonyms | MMP-9, MANDP2, GELB, Gelatinase B, CLG4B |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. Western blot analysis of MMP9 on rat spleen tissue lysate using anti-MMP9 antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MMP9 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-MMP9 antibody. Counter stained with hematoxylin. 4. ICC staining MMP9 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining MMP9 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. Flow cytometric analysis of A431 cells with MMP9 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
Application | |
Recommended Dose | WB: 1:500-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | Recombinant Antibody |
Purification | ProA affinity purified |
Appearance | Liquid |
Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
Research Background | The matrix metalloproteinases (MMPs) are a family of peptidase pathway responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin MMP-9 (also designated 92 kDa type IV collagenase or gelatinase B) has been shown to degrade bone collagens in concert with MMP-1 (also specified interstitial collagenase, fibroblast collagenase or Collagenase-1), and cysteine proteases and may play a role in bone osteoclastic resorption. MMP-1 is downregulated by p53, and abnormality of p53 expression can contribute to joint degradation in rheumatoid arthritis by regulating MMP-1 expression. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein |
Uniprot ID |
Molecular Weight | Theoretical: 100 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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