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Anti-MMP-9 Antibody (3R987)

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Catalog No. TMAB-01160
Alias MMP-9, MANDP2, GELB, Gelatinase B, CLG4B

Anti-MMP-9 Antibody (3R987) is a Rabbit antibody targeting MMP-9. Anti-MMP-9 Antibody (3R987) can be used in IF,IHC-Fr,IHC-P,WB.

Anti-MMP-9 Antibody (3R987)

Anti-MMP-9 Antibody (3R987)

😃Good
Catalog No. TMAB-01160Alias MMP-9, MANDP2, GELB, Gelatinase B, CLG4B
Anti-MMP-9 Antibody (3R987) is a Rabbit antibody targeting MMP-9. Anti-MMP-9 Antibody (3R987) can be used in IF,IHC-Fr,IHC-P,WB.
Pack SizePriceAvailabilityQuantity
25 μL$1547-10 days
50 μL$2737-10 days
100 μL$4877-10 days
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Product Introduction

Bioactivity
Description
Anti-MMP-9 Antibody (3R987) is a Rabbit antibody targeting MMP-9. Anti-MMP-9 Antibody (3R987) can be used in IF,IHC-Fr,IHC-P,WB.
SynonymsMMP-9, MANDP2, GELB, Gelatinase B, CLG4B
Ig Type
IgG
Clone
3R987
Reactivity
Human,Mouse,Rat
Verified Activity
1. 25 μg total protein per Lane of various lysates probed with MMP9 monoclonal antibody, unconjugated (TMAB-01160) at 1:5000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.
2. U2OS (H) cells were treated with or without TPA (200nM,48h) for 30 min, 25 μg total protein per Lane of cell lysates probed with MMP9 monoclonal antibody, unconjugated (TMAB-01160) at 1:5000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.
3. MDA-MB-231 (H) cells were treated with or without TPA (200nM,24h) for 30 min, 25 μg total protein per Lane of cell lysates probed with MMP9 monoclonal antibody, unconjugated (TMAB-01160) at 1:5000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.
4. Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MMP9 Monoclonal Antibody, Unconjugated (TMAB-01160) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MMP9 Monoclonal Antibody, Unconjugated (TMAB-01160) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MMP9 Monoclonal Antibody, Unconjugated (TMAB-01160) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MMP9 Monoclonal Antibody, Unconjugated (TMAB-01160) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MMP9 Monoclonal Antibody, Unconjugated (TMAB-01160) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MMP9 Monoclonal Antibody, Unconjugated (TMAB-01160) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MMP9 Monoclonal Antibody, Unconjugated (TMAB-01160) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
11. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MMP9 Monoclonal Antibody, Unconjugated (TMAB-01160) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
12. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MMP9 Monoclonal Antibody, Unconjugated (TMAB-01160) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
Application
Recommended Dose
WB: 1:500-5000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationSecreted; extracellular space; extracellular matrix.
Tissue SpecificityProduced by normal alveolar macrophages and granulocytes. Post-translational modifications : Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
ConstructionRecombinant Antibody
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1 mg/mL
Research BackgroundProteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq, Jul 2008].
Antigen Details
Immunogen
A synthesized peptide: human MMP9
Antigen Species
Human
Gene Name
Mmp9
Gene ID
Protein Name
Matrix metalloproteinase-9
Uniprot ID
Biology Area
MMP,Protease inhibitors ELISA kits,Cancer proteins ELISA kits,Atherosclerotic proteins ELISA kits,MMPs,Thrombosis,MMP,MMPs,MMPs,MMPs
Function
May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly- -Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
Chemical Properties
Molecular WeightTheoretical: 100 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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