Antibody Type: Recombinant Rabbit Monoclonal

Application: WB,IHC-P,IHC-Fr,IF

Reactivity: Human,Mouse,Rat

IgG

3R987

Human,Mouse,Rat

1. 25 μg total protein per Lane of various lysates probed with MMP9 monoclonal antibody, unconjugated (TMAB-01160) at 1:5000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.
2. U2OS (H) cells were treated with or without TPA (200nM,48h) for 30 min, 25 μg total protein per Lane of cell lysates probed with MMP9 monoclonal antibody, unconjugated (TMAB-01160) at 1:5000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.
3. MDA-MB-231 (H) cells were treated with or without TPA (200nM,24h) for 30 min, 25 μg total protein per Lane of cell lysates probed with MMP9 monoclonal antibody, unconjugated (TMAB-01160) at 1:5000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.
4. Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MMP9 Monoclonal Antibody, Unconjugated (TMAB-01160) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MMP9 Monoclonal Antibody, Unconjugated (TMAB-01160) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MMP9 Monoclonal Antibody, Unconjugated (TMAB-01160) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MMP9 Monoclonal Antibody, Unconjugated (TMAB-01160) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MMP9 Monoclonal Antibody, Unconjugated (TMAB-01160) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MMP9 Monoclonal Antibody, Unconjugated (TMAB-01160) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MMP9 Monoclonal Antibody, Unconjugated (TMAB-01160) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
11. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MMP9 Monoclonal Antibody, Unconjugated (TMAB-01160) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
12. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MMP9 Monoclonal Antibody, Unconjugated (TMAB-01160) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

WB,IHC-P,IHC-Fr,IF

WB: 1:500-5000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Monoclonal

A synthesized peptide: human MMP9

Human

4318

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