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Anti-INCENP Monoclonal Antibody (7L525)
(Synonyms: Inner centromere protein INCENP, Inner centromere protein antigens 135kD 155kD, Inner centromere protein antigens 135/155kDa, Inner centromere protein, INCENP, INCE_HUMAN, Chromosomal passenger protein, binds and activates aurora B and C in vivo and in vitro) Copy Product Info
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Synonyms: Inner centromere protein INCENP, Inner centromere protein antigens 135kD 155kD, Inner centromere protein antigens 135/155kDa, Inner centromere protein, INCENP, INCE_HUMAN, Chromosomal passenger protein, binds and activates aurora B and C in vivo and in vitro
Catalog No. TMAC-02158 Copy Product Info
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Anti-INCENP Monoclonal Antibody (7L525) is a Mouse antibody targeting INCENP. Anti-INCENP Monoclonal Antibody (7L525) can be used in IHC,IF,FCM.
Anti-INCENP Monoclonal Antibody
(7L525)
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 µL | $297 | 7-10 days | 7-10 days | |
| 100 µL | $428 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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| Description | Anti-INCENP Monoclonal Antibody (7L525) is a Mouse antibody targeting INCENP. Anti-INCENP Monoclonal Antibody (7L525) can be used in IHC,IF,FCM. |
| Synonyms | Inner centromere protein INCENP, Inner centromere protein antigens 135kD 155kD, Inner centromere protein antigens 135/155kDa, Inner centromere protein, INCENP, INCE_HUMAN, Chromosomal passenger protein, binds and activates aurora B and C in vivo and in vitro |
| Ig Type | IgG1 |
| Clone | 7L525 |
| Reactivity | Human |
| Verified Activity | 1. Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-INCENP antibody. Counter stained with hematoxylin. 2. Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-INCENP antibody. Counter stained with hematoxylin. 3. ICC staining INCENP (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. Flow cytometric analysis of Jurkat cells with INCENP antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).  , , , |
| Application | |
| Recommended Dose | IHC: 1/200-1/1000; IF: 1/200-1/1000; FCM: 1/200-1/400 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Purified by protein A or protein G |
| Appearance | Liquid |
| Formulation | PBS with 0.05% sodium azide |
| Concentration | 1 mg/mL |
| Research Background | In mammalian cells, 2 broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins and 'passenger,' or transiently interacting, proteins (reviewed by Choo, 1997). The constitutive proteins include CENPA (centromere protein A; MIM 117139), CENPB (MIM 117140), CENPC1 (MIM 117141), and CENPD (MIM 117142). The term 'passenger proteins' encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle (Earnshaw and Mackay, 1994 [PubMed 8088460]). These include CENPE (MIM 117143); MCAK (MIM 604538); KID (MIM 603213); cytoplasmic dynein (e.g., MIM 600112); CliPs (e.g., MIM 179838); and CENPF/mitosin (MIM 600236). The inner centromere proteins (INCENPs) (Earnshaw and Cooke, 1991 [PubMed 1860899]), the initial members of the passenger protein group, display a broad localization along chromosomes in the early stages of mitosis but gradually become concentrated at centromeres as the cell cycle progresses into mid-metaphase. During telophase, the proteins are located within the midbody in the intercellular bridge, where they are discarded after cytokinesis |
| Conjucates | Unconjugated |
| Immunogen | Purified recombinant fragment of human INCENP (AA: 369-583) expressed in E. Coli. |
| Antigen Species | Human |
| Gene Name | INCENP |
| Gene ID | |
| Protein Name | Inner centromere protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 105 kDa. |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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