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Anti-INCENP Antibody (3Z349)
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Catalog No. TMAC-02158
Alias Inner centromere protein INCENP, Inner centromere protein antigens 135kD 155kD, Inner centromere protein antigens 135/155kDa, Inner centromere protein, INCENP, INCE_HUMAN, Chromosomal passenger protein, binds and activates aurora B and C in vivo and in vitro
Anti-INCENP Antibody (3Z349) is a Mouse antibody targeting INCENP. Anti-INCENP Antibody (3Z349) can be used in WB,ICC,IHC,FCM.
Anti-INCENP Antibody (3Z349)
Anti-INCENP Antibody (3Z349)
😃Good
Catalog No. TMAC-02158Alias Inner centromere protein INCENP, Inner centromere protein antigens 135kD 155kD, Inner centromere protein antigens 135/155kDa, Inner centromere protein, INCENP, INCE_HUMAN, Chromosomal passenger protein, binds and activates aurora B and C in vivo and in vitro
Anti-INCENP Antibody (3Z349) is a Mouse antibody targeting INCENP. Anti-INCENP Antibody (3Z349) can be used in WB,ICC,IHC,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $428 | 7-10 days |
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| Description | Anti-INCENP Antibody (3Z349) is a Mouse antibody targeting INCENP. Anti-INCENP Antibody (3Z349) can be used in WB,ICC,IHC,FCM. |
| Synonyms | Inner centromere protein INCENP, Inner centromere protein antigens 135kD 155kD, Inner centromere protein antigens 135/155kDa, Inner centromere protein, INCENP, INCE_HUMAN, Chromosomal passenger protein, binds and activates aurora B and C in vivo and in vitro |
| Clone | 3Z349 |
| Reactivity | Human |
| Verified Activity | 1. Western blot analysis of INCENP on human INCENP recombinant protein using anti-INCENP antibody at 1/1,000 dilution. 2. Western blot analysis of INCENP on HEK293 (1) and INCENP-hIgGFc transfected HEK293 (2) cell lysate using anti-INCENP antibody at 1/1,000 dilution. 3. Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-INCENP antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-INCENP antibody. Counter stained with hematoxylin. 5. ICC staining INCENP (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. Flow cytometric analysis of Jurkat cells with INCENP antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red). |
| Application | |
| Recommended Dose | WB: 1:500-1000; IHC: 1:50-200; ICC: 1:100-500; FCM: 1:100-200 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
| Research Background | A replicated chromosome includes two kinetochores that control chromosome segregation during mitosis. The centromere proteins CENP-A, CENP-B, CENP-C, CENP-E, CENP-F (also designated mitosin), CENP-H and INCENP are kinetochore proteins that are involved in mitotic events. The centromere proteins are expressed at different levels throughout the cell cycle and are involved in the formation of the centromere and the organization and function of the kinetochore. INCENP, which also is designated inner centromere protein, is a chromosomal passenger protein that is crucial for chromosome segregation. During mitosis it is also required for cytokinesis onset. This protein, which can form a homodimer or a heterodimer, binds directly to microtubules and interacts with AURKB, AURKC, CBX3 and β Tubulin. This nuclear protein localizes to the mitotic spindle, methaphase chromosomes and during anaphase, to the equatorial cortex. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 105 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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