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Anti-IGF1R/CD221 Polyclonal Antibody 2
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Catalog No. TMAB-00919
Anti-IGF1R/CD221 Polyclonal Antibody 2 is a Rabbit antibody targeting IGF1R/CD221. Anti-IGF1R/CD221 Polyclonal Antibody 2 can be used in WB,FCM.
Anti-IGF1R/CD221 Polyclonal Antibody 2
Anti-IGF1R/CD221 Polyclonal Antibody 2
😃Good
Catalog No. TMAB-00919
Anti-IGF1R/CD221 Polyclonal Antibody 2 is a Rabbit antibody targeting IGF1R/CD221. Anti-IGF1R/CD221 Polyclonal Antibody 2 can be used in WB,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,FCM Reactivity: Human,Mouse (predicted:Rat,Pig,Cow,Sheep) |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Pig,Cow,Sheep) |
| Verified Activity | 1. Positive control: (mo) splenocytes (2% Paraformaldehyde-fixed) Isotype Control Antibody: Rabbit IgG; Dilution: 1 μg in 100 μL 1 X PBS containing 0.5% BSA Secondary Antibody: Goat anti-rabbit IgG-FITC; Dilution: 1:200 in 1 X PBS containing 0.5% BSA Primary Antibody: rabbit Anti-IGF1R TMAB-00919; Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA 2. Blank control (blue): Mouse Spleen (fixed with 2% paraformaldehyde for 10 min at 37°C). Primary Antibody: Rabbit Anti-IGF1R antibody (TMAB-00919,Green); Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit Igg (orange),used under the same conditions; Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. 3. Sample: Kidney (Mouse) Lysate at 40 μg Primary: Anti-IGF1R (TMAB-00919) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 69/150 kDa Observed band size: 69 kDa |
| Application | WB,FCM |
| Recommended Dose | WB: 1:500-2000; FCM: 1μg/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Membrane; Single-pass type I membrane protein. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This receptor binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. It has a tyrosine-protein kinase activity, which is necessary for the activation of the IGF1-stimulated downstream signaling cascade. When present in a hybrid receptor with INSR, binds IGF1. PubMed:12138094 shows that hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low affinity by IGF2 and not significantly activated by insulin, and that hybrid receptors composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2 and insulin. In contrast, PubMed:16831875 shows that hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, both bind IGF1 and have a low affinity for insulin. |
| Immunogen | KLH conjugated synthetic peptide: human IGF1R/CD221 beta chain |
| Antigen Species | Human |
| Gene ID | 3480 |
| Uniprot ID | |
| Biology Area | Insulin / Insulin-like,Aging,Insulin and insulin-like |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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