Antibody Type: Rabbit Polyclonal

Application: ICC/IF,FCM

Reactivity: Human (predicted:Mouse,Rat,Chicken,Pig)

IgG

Human (predicted:Mouse,Rat,Chicken,Pig)

1. The HepG2 (H) (treated with 500uM CoCl2 for 6 hours) cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C,the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at room temperature). Primary Antibody (green): Rabbit Anti-HIF-1 Alpha antibody (TMAB-00855): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC: 1 μg/test. Isotype Control (orange): Rabbit IgG. Blank control (black): PBS.
2. 4% Paraformaldehyde-fixed HepG2 (treated with 500uM CoCl2 for 6 hours)(H) cell; Triton X-100 at RT for 20 min; Antibody incubation with (HIF-1 Alpha) polyclonal Antibody, unconjugated (TMAB-00855) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nucleus. PBS instead of the primary antibody was used as the blank control.

ICC/IF,FCM

ICC/IF: 1:50-200; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: middle of human HIF-1 Alpha

Human

3091

HIF,Hydroxylases,HIF,Jumonji containing proteins,Methylation