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Anti-GSN Antibody (3E486)

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Catalog No. TMAC-01642

Anti-GSN Antibody (3E486) is a Rabbit antibody targeting GSN. Anti-GSN Antibody (3E486) can be used in WB,ICC,IF,IHC,FCM.

Anti-GSN Antibody (3E486)

Anti-GSN Antibody (3E486)

😃Good
Catalog No. TMAC-01642
Anti-GSN Antibody (3E486) is a Rabbit antibody targeting GSN. Anti-GSN Antibody (3E486) can be used in WB,ICC,IF,IHC,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2977-10 days
100 μL$4977-10 days
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Product Introduction

Bioactivity
Description
Anti-GSN Antibody (3E486) is a Rabbit antibody targeting GSN. Anti-GSN Antibody (3E486) can be used in WB,ICC,IF,IHC,FCM.
Ig Type
IgG
Clone
3E486
Reactivity
Human,Mouse
Verified Activity
1. Western blot analysis of Gelsolin on different lysates using anti-Gelsolin antibody at 1/500 dilution. Positive control: Lane 1: Mouse thymus, Lane 2: Mouse lung, Lane 3: THP-1, Lane 4: HepG2.
2. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Gelsolin antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Gelsolin antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Gelsolin antibody. Counter stained with hematoxylin.
5. ICC staining Gelsolin in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. ICC staining Gelsolin in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. Flow cytometric analysis of THP-1 cells with Gelsolin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Application
Recommended Dose
WB: 1:500-2000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundGelsolin (also known as brevin, Actin-depolymerizing factor or ADF), a protein of leukocytes, platelets and other cells, severs Actin filaments in the presence of submicromolar calcium, thereby isolating cytoplasmic Actin gels. A calcium-independent mechanism reverses the process. A Gelsolin variant with 23 more amino-terminal amino acids is a plasma component probably involved in the clearance of Actin, the most abundant human protein, from the circulation. It has been suggested that a single gene encodes both cell and plasma gelsolins. Gelsolin may be unique in that it is made for both secretion and intracytoplasmic location. Amino acid homology was identified between Gelsolin and the amyloid of the Finnish variety of amyloidosis. The amyloid in this disorder is antigenically and structurally related to Gelsolin. Gelsolin is the principal intracellular and extracellular Actin-severing protein. Gelsolin and Gc protein together constitute the extracellular Actin-scavenger system which prevents the toxic effects of Actin release into the extracellular space under circumstances of cell necrosis.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 86 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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