Anti-FZD7 Polyclonal Antibody is a Rabbit antibody targeting FZD7. Anti-FZD7 Polyclonal Antibody can be used in WB,FCM.

IgG

Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,GuineaPig)

1. Blank control: A431. Primary Antibody (green line): Rabbit Anti-Frizzled 7 antibody (TMAB-00723)
Dilution: 3 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 3 μg/test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
2. Sample:
Muscle (Mouse) Lysate at 40 μg
Primary: Anti-Frizzled 7 (TMAB-00723) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa
3. Sample:
HepG2 (Human) Cell Lysate at 30 μg
Muscle (Mouse) Lysate at 40 μg
Muscle (Rat) Lysate at 40 μg
Primary: Anti-Frizzled 7 (TMAB-00723) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa

WB,FCM

WB: 1:500-2000; FCM: 3ug/Test

Polyclonal

KLH conjugated synthetic peptide: human Frizzled 7

Human