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Anti-ERCC1 Antibody (7K54)
😃Good
Catalog No. TMAC-01359
Alias UV20, UV 20, RAD10, RAD 10, Excision repair protein, Excision Repair Cross Complementing Rodent Repair Deficiency Complementation Group 1, Excision repair cross complementing 1, Excision repair cross complementation group 1, ERCC1_HUMAN, ERCC1, ERCC 1, DNA excision repair protein ERCC-1, DNA excision repair protein ERCC1, DNA excision repair protein ERCC 1, COFS4, COFS 4
Anti-ERCC1 Antibody (7K54) is a Rabbit antibody targeting ERCC1. Anti-ERCC1 Antibody (7K54) can be used in WB,ICC/IF,IHC.
Anti-ERCC1 Antibody (7K54)
Anti-ERCC1 Antibody (7K54)
😃Good
Catalog No. TMAC-01359Alias UV20, UV 20, RAD10, RAD 10, Excision repair protein, Excision Repair Cross Complementing Rodent Repair Deficiency Complementation Group 1, Excision repair cross complementing 1, Excision repair cross complementation group 1, ERCC1_HUMAN, ERCC1, ERCC 1, DNA excision repair protein ERCC-1, DNA excision repair protein ERCC1, DNA excision repair protein ERCC 1, COFS4, COFS 4
Anti-ERCC1 Antibody (7K54) is a Rabbit antibody targeting ERCC1. Anti-ERCC1 Antibody (7K54) can be used in WB,ICC/IF,IHC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $298 | 7-10 days | |
| 100 μL | $496 | 7-10 days |
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| Description | Anti-ERCC1 Antibody (7K54) is a Rabbit antibody targeting ERCC1. Anti-ERCC1 Antibody (7K54) can be used in WB,ICC/IF,IHC. |
| Synonyms | UV20, UV 20, RAD10, RAD 10, Excision repair protein, Excision Repair Cross Complementing Rodent Repair Deficiency Complementation Group 1, Excision repair cross complementing 1, Excision repair cross complementation group 1, ERCC1_HUMAN, ERCC1, ERCC 1, DNA excision repair protein ERCC-1, DNA excision repair protein ERCC1, DNA excision repair protein ERCC 1, COFS4, COFS 4 |
| Ig Type | IgG |
| Clone | 7K54 |
| Reactivity | Human,Mouse |
| Verified Activity | 1. Western blot analysis of ERCC1 on different lysates using anti-ERCC1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: Human lung, Lane 3: 293T, Lane 4: A549. 2. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ERCC1 antibody. Counter stained with hematoxylin. 3. ICC staining ERCC1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining ERCC1 in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining ERCC1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining ERCC1 in PANC-1 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining ERCC1 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
| Application | |
| Recommended Dose | WB: 1:1000; IHC: 1:50-100; ICC/IF: 1:50 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Xeroderma pigmentosum (XP) is an autosomal recessive disorder characterized by a genetic predisposition to sunlight-induced skin cancer; it is commonly due to deficiencies in DNA repair enzymes. The most frequent mutations are found in the XP genes from group A through G and group V, which encode for nucleotide excision repair proteins. XPF, which is also designated ERCC4 or ERCC11, associates directly with the excision repair cross-complementing 1 (ERCC1) factor. ERCC-1, a functional homolog of Rad10 in S. cerevisiae, is a component of a structure-specific endonuclease that is responsible for 5' incisions during DNA repair. The ERCC1-XPF endo-nuclease preferentially cleaves one strand of DNA between duplex and single-stranded regions near borders of the stem-loop structure and, thereby, contributes to the initial steps of the nucleotide excision repair process. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 36 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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