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Anti-CRISPR-Cas9 Antibody (4K140)

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Catalog No. TMAC-00979
Alias SpyCas9

Anti-CRISPR-Cas9 Antibody (4K140) is a Rabbit antibody targeting CRISPR-Cas9. Anti-CRISPR-Cas9 Antibody (4K140) can be used in WB,IHC,ICC/IF,FCM.

Anti-CRISPR-Cas9 Antibody (4K140)

Anti-CRISPR-Cas9 Antibody (4K140)

😃Good
Catalog No. TMAC-00979Alias SpyCas9
Anti-CRISPR-Cas9 Antibody (4K140) is a Rabbit antibody targeting CRISPR-Cas9. Anti-CRISPR-Cas9 Antibody (4K140) can be used in WB,IHC,ICC/IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2977-10 days
100 μL$4977-10 days
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Product Introduction

Bioactivity
Description
Anti-CRISPR-Cas9 Antibody (4K140) is a Rabbit antibody targeting CRISPR-Cas9. Anti-CRISPR-Cas9 Antibody (4K140) can be used in WB,IHC,ICC/IF,FCM.
AliasSpyCas9
Ig Type
IgG
Reactivity
Recombinant Cas9 Streptococcus pyogenes
Application
Recommended Dose
WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundCRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant fragment derived from Streptococcus pyogenes
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 158 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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