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Anti-CRISPR-Cas9 Antibody (4K140)
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Catalog No. TMAC-00979
Alias SpyCas9
Anti-CRISPR-Cas9 Antibody (4K140) is a Rabbit antibody targeting CRISPR-Cas9. Anti-CRISPR-Cas9 Antibody (4K140) can be used in FCM,ICC/IF,IHC,WB.
Anti-CRISPR-Cas9 Antibody (4K140)
Anti-CRISPR-Cas9 Antibody (4K140)
😃Good
Catalog No. TMAC-00979Alias SpyCas9
Anti-CRISPR-Cas9 Antibody (4K140) is a Rabbit antibody targeting CRISPR-Cas9. Anti-CRISPR-Cas9 Antibody (4K140) can be used in FCM,ICC/IF,IHC,WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
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Chemical Properties
| Description | Anti-CRISPR-Cas9 Antibody (4K140) is a Rabbit antibody targeting CRISPR-Cas9. Anti-CRISPR-Cas9 Antibody (4K140) can be used in FCM,ICC/IF,IHC,WB. |
| Synonyms | SpyCas9 |
| Ig Type | IgG |
| Clone | 4K140 |
| Reactivity | Recombinant Cas9 Streptococcus pyogenes |
| Verified Activity | 1. Western blot analysis of CRISPR-Cas9 SP expression in (1) 293T cell lysate transfected with CRISPR-Cas9; (2) 293T cell lysate; (3) 3T3 cell lysate; (4) PC12 cell lysate. 2. Immunofluorescent analysis of 293T cells transfected with CRISPR-SpCas9, using CRISPR-Cas9 SP Antibody . |
| Application | |
| Recommended Dose | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant fragment derived from Streptococcus pyogenes |
| Uniprot ID |
| Molecular Weight | Theoretical: 158 kDa. |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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