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Anti-Collagen II/COL2A1 Polyclonal Antibody is a Rabbit antibody targeting Collagen II/COL2A1. Anti-Collagen II/COL2A1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $221 | 7-10 days | |
100 μL | $373 | 7-10 days | |
200 μL | $529 | 7-10 days |
Description | Anti-Collagen II/COL2A1 Polyclonal Antibody is a Rabbit antibody targeting Collagen II/COL2A1. Anti-Collagen II/COL2A1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
Alias | STL1, SEDC, collagen, type II, α1, collagen, type II, alpha 1, COL11A3, AOM, ANFH |
Ig Type | IgG |
Reactivity | Human,Rabbit (predicted:Mouse,Rat,Chicken,Dog,Pig,Cow,GuineaPig) |
Verified Activity | 1. Sample: 293T (human) cell Lysate at 40 μg A549 (human) cell Lysate at 40 μg MCF-7 (human) cell Lysate at 40 μg HepG2 (human) cell Lysate at 40 μg Placenta (mouse) Lysate at 40 μg MG63 (human) cell Lysate at 40 μg Primary: Anti-Collagen II (TMAB-00456) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 117 kDa Observed band size: 120 kDa 2. Tissue/cell: human esophageal carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Collagen II Polyclonal Antibody, Unconjugated (TMAB-00456) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 3. Tissue/cell: rabbit meniscus tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Collagen II Polyclonal Antibody, Unconjugated (TMAB-00456) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 μg/ml,blue) was used to stain the cell nucleus. |
Application | WB,IHC-P,IHC-Fr,IF |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Secreted, extracellular space, extracellular matrix. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene. [provided by RefSeq, Jul 2008] |
Immunogen | KLH conjugated synthetic peptide: human Collagen II |
Antigen Species | Human |
Gene ID | |
Uniprot ID |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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