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Anti-Collagen II/COL2A1 Polyclonal Antibody

😃Good
Catalog No. TMAB-00456
Alias STL1, SEDC, collagen, type II, α1, collagen, type II, alpha 1, COL11A3, AOM, ANFH

Anti-Collagen II/COL2A1 Polyclonal Antibody is a Rabbit antibody targeting Collagen II/COL2A1. Anti-Collagen II/COL2A1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-Collagen II/COL2A1 Polyclonal Antibody

Anti-Collagen II/COL2A1 Polyclonal Antibody

😃Good
Catalog No. TMAB-00456Alias STL1, SEDC, collagen, type II, α1, collagen, type II, alpha 1, COL11A3, AOM, ANFH
Anti-Collagen II/COL2A1 Polyclonal Antibody is a Rabbit antibody targeting Collagen II/COL2A1. Anti-Collagen II/COL2A1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2217-10 days
100 μL$3737-10 days
200 μL$5297-10 days
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Product Introduction

Bioactivity
Description
Anti-Collagen II/COL2A1 Polyclonal Antibody is a Rabbit antibody targeting Collagen II/COL2A1. Anti-Collagen II/COL2A1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
AliasSTL1, SEDC, collagen, type II, α1, collagen, type II, alpha 1, COL11A3, AOM, ANFH
Ig Type
IgG
Reactivity
Human,Rabbit (predicted:Mouse,Rat,Chicken,Dog,Pig,Cow,GuineaPig)
Verified Activity
1. Sample:
293T (human) cell Lysate at 40 μg
A549 (human) cell Lysate at 40 μg
MCF-7 (human) cell Lysate at 40 μg
HepG2 (human) cell Lysate at 40 μg
Placenta (mouse) Lysate at 40 μg
MG63 (human) cell Lysate at 40 μg
Primary: Anti-Collagen II (TMAB-00456) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 117 kDa
Observed band size: 120 kDa
2. Tissue/cell: human esophageal carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Collagen II Polyclonal Antibody, Unconjugated (TMAB-00456) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Tissue/cell: rabbit meniscus tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Collagen II Polyclonal Antibody, Unconjugated (TMAB-00456) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 μg/ml,blue) was used to stain the cell nucleus.
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationSecreted, extracellular space, extracellular matrix.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene. [provided by RefSeq, Jul 2008]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Collagen II
Antigen Species
Human
Gene ID
Uniprot ID
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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