Anti-Collagen II/COL2A1 Polyclonal Antibody is a Rabbit antibody targeting Collagen II/COL2A1. Anti-Collagen II/COL2A1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Rabbit (predicted:Mouse,Rat,Chicken,Dog,Pig,Cow,GuineaPig)

1. Sample:
293T (human) cell Lysate at 40 μg
A549 (human) cell Lysate at 40 μg
MCF-7 (human) cell Lysate at 40 μg
HepG2 (human) cell Lysate at 40 μg
Placenta (mouse) Lysate at 40 μg
MG63 (human) cell Lysate at 40 μg
Primary: Anti-Collagen II (TMAB-00456) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 117 kDa
Observed band size: 120 kDa
2. Tissue/cell: human esophageal carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Collagen II Polyclonal Antibody, Unconjugated (TMAB-00456) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Tissue/cell: rabbit meniscus tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Collagen II Polyclonal Antibody, Unconjugated (TMAB-00456) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 μg/ml,blue) was used to stain the cell nucleus.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Collagen II

Human

COL2A1

Procollagen Type II N-Terminal Propeptide

Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.