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Anti-Coagulation Factor VIII/FVIII/F8 Polyclonal Antibody
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Catalog No. TMAB-00453
Anti-Coagulation Factor VIII/FVIII/F8 Polyclonal Antibody is a Rabbit antibody targeting Coagulation Factor VIII/FVIII/F8. Anti-Coagulation Factor VIII/FVIII/F8 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Anti-Coagulation Factor VIII/FVIII/F8 Polyclonal Antibody
Anti-Coagulation Factor VIII/FVIII/F8 Polyclonal Antibody
😃Good
Catalog No. TMAB-00453
Anti-Coagulation Factor VIII/FVIII/F8 Polyclonal Antibody is a Rabbit antibody targeting Coagulation Factor VIII/FVIII/F8. Anti-Coagulation Factor VIII/FVIII/F8 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: IHC-P,IHC-Fr,IF Reactivity: Human,Mouse,Rat (predicted:Dog,Rabbit) |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Dog,Rabbit) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (factor VIII) Polyclonal Antibody, Unconjugated (TMAB-00453) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Factor VIII Polyclonal Antibody, Unconjugated (TMAB-00453) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. |
| Application | IHC-P,IHC-Fr,IF |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted, extracellular space. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene encodes coagulation factor VIII, which participates in the intrinsic pathway of blood coagulation; factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein, isoform a, which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein, isoform b, which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. Defects in this gene results in hemophilia A, a common recessive X-linked coagulation disorder. [provided by RefSeq, Jul 2008]. |
| Immunogen | KLH conjugated synthetic peptide: rat factor VIII |
| Antigen Species | Rat |
| Biology Area | Intrinsic |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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