Anti-CD80 Polyclonal Antibody 2 is a Rabbit antibody targeting CD80. Anti-CD80 Polyclonal Antibody 2 can be used in WB,FCM.

IgG

Human,Mouse,Rat

1. Sample:
Lane 1: Raw264.7 (Mouse) Cell Lysate at 30 μg
Lane 2: SP2/0 (Mouse) Cell Lysate at 30 μg
Lane 3: Thymus (Rat) Lysate at 40 μg
Lane 4: Raji (Human) Cell Lysate at 30 μg
Lane 5: K562 (Human) Cell Lysate at 30 μg
Primary: Anti-CD80 (TMAB-00393) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kDa
Observed band size: 60 kDa
2. Blank control: U937 (blue). Primary Antibody: Rabbit Anti-CD80 antibody (TMAB-00393), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange),used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (TMAB-00393, 1 μg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice.
3. Blank control: Raji.
Primary Antibody (green line): Rabbit Anti-CD80 antibody (TMAB-00393)
Dilution: 2 μg/Test;
Secondary Antibody: Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

WB,FCM

WB: 1:500-2000; FCM: 2µg/Test

Polyclonal

KLH conjugated synthetic peptide: rat CD80

Rat

CD,B Lymphocytic Lineage,Dendritic Cell Lineage,Monocytic Lineage