Anti-CD80 Polyclonal Antibody 2 is a Rabbit antibody targeting CD80. Anti-CD80 Polyclonal Antibody 2 can be used in FCM,WB.

IgG

Human,Mouse,Rat

1. Sample:
Lane 1: Raw264.7 (Mouse) Cell Lysate at 30 μg
Lane 2: SP2/0 (Mouse) Cell Lysate at 30 μg
Lane 3: Thymus (Rat) Lysate at 40 μg
Lane 4: Raji (Human) Cell Lysate at 30 μg
Lane 5: K562 (Human) Cell Lysate at 30 μg
Primary: Anti-CD80 (TMAB-00393) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kDa
Observed band size: 60 kDa
2. Blank control: Raji.
Primary Antibody (green line): Rabbit Anti-CD80 antibody (TMAB-00393)
Dilution: 2 μg/Test;
Secondary Antibody: Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

WB: 1:500-2000; FCM: 2µg/Test

Polyclonal

KLH conjugated synthetic peptide: rat CD80

Rat

CD80

T-lymphocyte activation antigen CD80

CD,B Lymphocytic Lineage,Dendritic Cell Lineage,Monocytic Lineage

Involved in the costimulatory signal essential for T lymphocytes activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.