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Anti-Cathepsin K Polyclonal Antibody

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Catalog No. TMAB-00298
Alias CTSO2, CTSO, CTSK, Cathepsin X, Cathepsin O2, Cathepsin O, Cathepsin K

Anti-Cathepsin K Polyclonal Antibody is a Rabbit antibody targeting Cathepsin K. Anti-Cathepsin K Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-Cathepsin K Polyclonal Antibody

Anti-Cathepsin K Polyclonal Antibody

😃Good
Catalog No. TMAB-00298Alias CTSO2, CTSO, CTSK, Cathepsin X, Cathepsin O2, Cathepsin O, Cathepsin K
Anti-Cathepsin K Polyclonal Antibody is a Rabbit antibody targeting Cathepsin K. Anti-Cathepsin K Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2227-10 days
100 μL$3737-10 days
200 μL$5287-10 days
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Product Introduction

Bioactivity
Description
Anti-Cathepsin K Polyclonal Antibody is a Rabbit antibody targeting Cathepsin K. Anti-Cathepsin K Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
AliasCTSO2, CTSO, CTSK, Cathepsin X, Cathepsin O2, Cathepsin O, Cathepsin K
Ig Type
IgG
Reactivity
Mouse,Rat (predicted:Human,Dog,Pig,Cow,Horse,GuineaPig)
Verified Activity
1. Tissue/cell: mouse heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Cathepsin K Polyclonal Antibody, Unconjugated (TMAB-00298) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample:
Lane 1: Mouse Raw264.7 cell lysates
Lane 2: Mouse 4T1 cell lysates
Primary: Anti-Cathepsin K (TMAB-00298) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 46 kDa
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationLysosome.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe protein encoded by this gene is a lysosomal cysteine proteinase involved in bone remodeling and resorption. This protein, which is a member of the peptidase C1 protein family, is predominantly expressed in osteoclasts. However, the encoded protein is also expressed in a significant fraction of human breast cancers, where it could contribute to tumor invasiveness. Mutations in this gene are the cause of pycnodysostosis, an autosomal recessive disease characterized by osteosclerosis and short stature. This gene may be subject to RNA editing. [provided by RefSeq, Jul 2008].
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Cathepsin K
Antigen Species
Human
Gene ID
Uniprot ID
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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