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Anti-Carbonic Anhydrase 12 Antibody (3Q864)
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Catalog No. TMAY-01030
Alias HsT18816, FLJ20151, CAXII, carbonic anhydrase XII, CA12
Anti-Carbonic Anhydrase 12 Antibody (3Q864) is a Mouse antibody targeting Carbonic Anhydrase 12. Anti-Carbonic Anhydrase 12 Antibody (3Q864) can be used in ICC/IF.
Anti-Carbonic Anhydrase 12 Antibody (3Q864)
Anti-Carbonic Anhydrase 12 Antibody (3Q864)
😃Good
Catalog No. TMAY-01030Alias HsT18816, FLJ20151, CAXII, carbonic anhydrase XII, CA12
Anti-Carbonic Anhydrase 12 Antibody (3Q864) is a Mouse antibody targeting Carbonic Anhydrase 12. Anti-Carbonic Anhydrase 12 Antibody (3Q864) can be used in ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $198 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: ICC/IF Reactivity: Human |
| Alias | HsT18816, FLJ20151, CAXII, carbonic anhydrase XII, CA12 |
| Ig Type | Mouse IgG1 |
| Clone | 3Q864 |
| Reactivity | Human |
| Verified Activity | Immunofluorescence staining of Human CA12 in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human CA12 monoclonal antibody (1:60). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope), countstained with DAPI (blue). Positive staining was localized to cytomembrane. |
| Application | ICC/IF |
| Recommended Dose | ICC-IF: 1:20-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Carbonic Anhydrase XII / CA12 (rh Carbonic Anhydrase XII / CA12; TMPY-00694; NP_001209.1; Met1-Gln291). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS with 10% Trehalose, pH7.0 |
| Research Background | Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CA12, also known as Car12 and carbonic anhydrase XII, is a type I membrane enzyme of an N-terminal extracellular catalytic domain, a membrane-spanning α-helix, and a small intracellular C-terminal domain. It is highly expressed in colon, kidney, prostate, intestine and activated lymphocytes and moderately expressed in pancreas, ovary, and testis. Overexpression of the CA12 is observed in certain human cancers and is used as a tumor marker. rmCA12 corresponds to the extracellular domain and has both carbonic anhydrase activity and esterase activity. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human Carbonic Anhydrase XII / CA12 protein (TMPY-00694) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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