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Anti-ALDH1A1 Polyclonal Antibody
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Catalog No. TMAB-00092
Anti-ALDH1A1 Polyclonal Antibody is a Rabbit antibody targeting ALDH1A1. Anti-ALDH1A1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-ALDH1A1 Polyclonal Antibody
Anti-ALDH1A1 Polyclonal Antibody
😃Good
Catalog No. TMAB-00092
Anti-ALDH1A1 Polyclonal Antibody is a Rabbit antibody targeting ALDH1A1. Anti-ALDH1A1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P,IHC-Fr,IF Reactivity: Human,Mouse (predicted:Rat,Cow,Horse,Rabbit,Sheep,Pig) |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Cow,Horse,Rabbit,Sheep,Pig) |
| Verified Activity | 1. Sample: Placenta (Mouse) Lysate at 40 μg Primary: Anti-ALDH1 (TMAB-00092) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kDa Observed band size: 54 kDa 2. Paraformaldehyde-fixed, paraffin embedded (human lung cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ALDH1) Polyclonal Antibody, Unconjugated (TMAB-00092) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (human liver cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ALDH1) Polyclonal Antibody, Unconjugated (TMAB-00092) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 4. Sample: A549 Cell (Human) Lysate at 40 μg HepG2 Cell (Human) Lysate at 40 μg Primary: Anti-ALDH1 (TMAB-00092) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kDa Observed band size: 54 kDa 5. Sample: Liver (mouse) Lysate at 40 μg Primary: Anti-ALDH1 (TMAB-00092) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kDa Observed band size: 54 kDa |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The protein encoded by this gene belongs to the aldehyde dehydrogenase family. Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. There are two major aldehyde dehydrogenase isozymes in the liver, cytosolic and mitochondrial, which are encoded by distinct genes, and can be distinguished by their electrophoretic mobility, kinetic properties, and subcellular localization. This gene encodes the cytosolic isozyme. Studies in mice show that through its role in retinol metabolism, this gene may also be involved in the regulation of the metabolic responses to high-fat diet. [provided by RefSeq, Mar 2011]. |
| Immunogen | KLH conjugated synthetic peptide: human ALDH1 |
| Antigen Species | Human |
| Gene ID | 216 |
| Uniprot ID | |
| Biology Area | Tumor biomarkers,Soma marker,Parkinson's disease,Neurogenesis,Intracellular Molecules |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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