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Anti-ADIPOR1 Polyclonal Antibody
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Catalog No. TMAB-00064
Alias TESBP1A, Progestin and adipoQ receptor family member I, Progestin and adipoQ receptor family member 1, PAQR1, ADIPOR1, Adiponectin receptor protein 1
Anti-ADIPOR1 Polyclonal Antibody is a Rabbit antibody targeting ADIPOR1. Anti-ADIPOR1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-ADIPOR1 Polyclonal Antibody
Anti-ADIPOR1 Polyclonal Antibody
😃Good
Catalog No. TMAB-00064Alias TESBP1A, Progestin and adipoQ receptor family member I, Progestin and adipoQ receptor family member 1, PAQR1, ADIPOR1, Adiponectin receptor protein 1
Anti-ADIPOR1 Polyclonal Antibody is a Rabbit antibody targeting ADIPOR1. Anti-ADIPOR1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-ADIPOR1 Polyclonal Antibody is a Rabbit antibody targeting ADIPOR1. Anti-ADIPOR1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | TESBP1A, Progestin and adipoQ receptor family member I, Progestin and adipoQ receptor family member 1, PAQR1, ADIPOR1, Adiponectin receptor protein 1 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Chicken,Dog,Rabbit) |
| Verified Activity | 1. Tissue/cell: human rectal tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Adiponectin Receptor 1 Polyclonal Antibody, Unconjugated (TMAB-00064) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Adiponectin Receptor 1 Polyclonal Antibody, Unconjugated (TMAB-00064) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 3. Sample: Muscle (Mouse) Lysate at 40 μg Primary: Anti-Adiponectin Receptor 1 (TMAB-00064) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kDa Observed band size: 42 kDa 4. Sample: Lane 1: Mouse Muscle tissue lysates Lane 2: Mouse Breast tissue lysates Lane 3: Mouse Heart tissue lysates Lane 4: Rat Muscle tissue lysates Lane 5: Rat Heart tissue lysates Primary: Anti-Adiponectin Receptor 1 (TMAB-00064) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kDa Observed band size: 42 kDa |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Membrane; Multi-pass membrane protein. Note=Localized to the cell membrane and intracellular organelles. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Acrp30 is a hormone secreted by adipocytes that acts as an antidiabetic and anti-atherogenic adipokine. Levels of adiponectin in the blood are decreased under conditions of obesity, insulin resistance and type 2 diabetes. Administration of adiponectin causes glucose-lowering effects and ameliorates insulin resistance in mice. Conversely, adiponectin-deficient mice exhibit insulin resistance and diabetes. This insulin-sensitizing effect of adiponectin seems to be mediated by an increase in fatty-acid oxidation through activation of AMP kinase and PPAR- . Cloning of complementary DNAs encoding adiponectin receptors 1 and 2 (AdipoR1 and AdipoR2) have shown that AdipoR1 is abundantly expressed in skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. |
| Immunogen | KLH conjugated synthetic peptide: human Adiponectin Receptor 1 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Acrp,Hormones |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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