Anti-ADIPOR1 Polyclonal Antibody is a Rabbit antibody targeting ADIPOR1. Anti-ADIPOR1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Chicken,Dog,Rabbit)

1. Tissue/cell: human rectal tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Adiponectin Receptor 1 Polyclonal Antibody, Unconjugated (TMAB-00064) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Adiponectin Receptor 1 Polyclonal Antibody, Unconjugated (TMAB-00064) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Sample: Muscle (Mouse) Lysate at 40 μg
Primary: Anti-Adiponectin Receptor 1 (TMAB-00064) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
4. Sample:
Lane 1: Mouse Muscle tissue lysates
Lane 2: Mouse Breast tissue lysates
Lane 3: Mouse Heart tissue lysates
Lane 4: Rat Muscle tissue lysates
Lane 5: Rat Heart tissue lysates
Primary: Anti-Adiponectin Receptor 1 (TMAB-00064) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa

WB,IHC-P,IHC-Fr,IF

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Adiponectin Receptor 1

Human

Acrp,Hormones