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Anti-ADIPOR1 Polyclonal Antibody

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Catalog No. TMAB-00064

Anti-ADIPOR1 Polyclonal Antibody is a Rabbit antibody targeting ADIPOR1. Anti-ADIPOR1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-ADIPOR1 Polyclonal Antibody

Anti-ADIPOR1 Polyclonal Antibody

😃Good
Catalog No. TMAB-00064
Anti-ADIPOR1 Polyclonal Antibody is a Rabbit antibody targeting ADIPOR1. Anti-ADIPOR1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$221 7-10 days
100 μL$372 7-10 days
200 μL$527 7-10 days
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Product Introduction

Bioactivity
Description
Anti-ADIPOR1 Polyclonal Antibody is a Rabbit antibody targeting ADIPOR1. Anti-ADIPOR1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Chicken,Dog,Rabbit)
Verified Activity
1. Tissue/cell: human rectal tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Adiponectin Receptor 1 Polyclonal Antibody, Unconjugated (TMAB-00064) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Adiponectin Receptor 1 Polyclonal Antibody, Unconjugated (TMAB-00064) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Sample: Muscle (Mouse) Lysate at 40 μg
Primary: Anti-Adiponectin Receptor 1 (TMAB-00064) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
4. Sample:
Lane 1: Mouse Muscle tissue lysates
Lane 2: Mouse Breast tissue lysates
Lane 3: Mouse Heart tissue lysates
Lane 4: Rat Muscle tissue lysates
Lane 5: Rat Heart tissue lysates
Primary: Anti-Adiponectin Receptor 1 (TMAB-00064) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationMembrane; Multi-pass membrane protein. Note=Localized to the cell membrane and intracellular organelles.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundAcrp30 is a hormone secreted by adipocytes that acts as an antidiabetic and anti-atherogenic adipokine. Levels of adiponectin in the blood are decreased under conditions of obesity, insulin resistance and type 2 diabetes. Administration of adiponectin causes glucose-lowering effects and ameliorates insulin resistance in mice. Conversely, adiponectin-deficient mice exhibit insulin resistance and diabetes. This insulin-sensitizing effect of adiponectin seems to be mediated by an increase in fatty-acid oxidation through activation of AMP kinase and PPAR- . Cloning of complementary DNAs encoding adiponectin receptors 1 and 2 (AdipoR1 and AdipoR2) have shown that AdipoR1 is abundantly expressed in skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Adiponectin Receptor 1
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Acrp,Hormones
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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