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Anti-Adiponectin Polyclonal Antibody
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Catalog No. TMAB-00063
Anti-Adiponectin Polyclonal Antibody is a Rabbit antibody targeting Adiponectin. Anti-Adiponectin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Adiponectin Polyclonal Antibody
Anti-Adiponectin Polyclonal Antibody
😃Good
Catalog No. TMAB-00063
Anti-Adiponectin Polyclonal Antibody is a Rabbit antibody targeting Adiponectin. Anti-Adiponectin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P,IHC-Fr,IF Reactivity: Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Rabbit) |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Rabbit) |
| Verified Activity | 1. Sample: muscle (Mouse) Lysate at 40 μg Primary: Anti-Adiponectin (TMAB-00063) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kDa Observed band size: 30 kDa 2. Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Adiponectin Polyclonal Antibody, Unconjugated (TMAB-00063) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 3. Tissue/cell: mouse liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Adiponectin Polyclonal Antibody, Unconjugated (TMAB-00063) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 4. Sample: Liver (Rat) Lysate at 40 μg Intestine (Mouse) Lysate at 40 μg Primary: Anti-Adiponectin (TMAB-00063) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (secondary antibody) at 1/5000 dilution Predicted band size: 25 kDa Observed band size: 25 kDa 5. Sample: Heart (Mouse) Lysate at 40 μg Primary: Anti-Adiponectin (TMAB-00063) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (secondary antibody) at 1/5000 dilution Predicted band size: 25 kDa Observed band size: 27 kDa |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Adiponectin: A protein hormone produced and secreted exclusively by adipocytes (fat cells) that regulates the metabolism of lipids and glucose. Adiponectin influences the body's response to insulin. Adiponectin also has antiinflammatory effects on the cells lining the walls of blood vessels. High blood levels of adiponectin are associated with a reduced risk of heart attack. Low levels of adiponectin are found in people who are obese (and who are at increased risk of a heart attack). Adipocytes produce and secrete a number of proteins, including leptin, adipsin, properdin, and tumor necrosis factor (TNF). An adipose tissue-specific factor was isolated and termed APM1 (AdiPose Most abundant gene transcript 1). The protein product of APM1 is adiponectin. The APM1 gene maps to chromosome 3q27. |
| Immunogen | KLH conjugated synthetic peptide: human Adiponectin |
| Antigen Species | Human |
| Gene ID | 9370 |
| Uniprot ID | |
| Biology Area | Response to hypoxia,Diabetes associated,Acrp,Hypoxia,Diabetes,Heart disease,Metabolic disorders,Obesity,Metabolism,Hormones,Adipogenesis |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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