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Anhydroleucovorin

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Catalog No. T205953Cas No. 7444-29-3
Alias Methyltetrahydrofolic Acid, Methenyltetrahydrofolic Acid, 5,10-Methenyltetrahydrofolic Acid

Anhydroleucovorin is the predominant, biologically active form of vitamin B9 (folate) that is utilized by the body for a multitude of vital cellular functions, including DNA synthesis, repair, and methylation, as well as the production of key neurotransmitters; Anhydroleucovorin plays an essential metabolic role in the remethylation pathway, converting the amino acid homocysteine to methionine, and serves as the main folate form found in systemic circulation and transported into tissues for physiological use.

Anhydroleucovorin

Anhydroleucovorin

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🥰Excellent
Catalog No. T205953Alias Methyltetrahydrofolic Acid, Methenyltetrahydrofolic Acid, 5,10-Methenyltetrahydrofolic AcidCas No. 7444-29-3
Anhydroleucovorin is the predominant, biologically active form of vitamin B9 (folate) that is utilized by the body for a multitude of vital cellular functions, including DNA synthesis, repair, and methylation, as well as the production of key neurotransmitters; Anhydroleucovorin plays an essential metabolic role in the remethylation pathway, converting the amino acid homocysteine to methionine, and serves as the main folate form found in systemic circulation and transported into tissues for physiological use.
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Batch Information

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Appearance:Solid
Color:Yellow
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Product Introduction

Bioactivity
Description
Anhydroleucovorin is the predominant, biologically active form of vitamin B9 (folate) that is utilized by the body for a multitude of vital cellular functions, including DNA synthesis, repair, and methylation, as well as the production of key neurotransmitters; Anhydroleucovorin plays an essential metabolic role in the remethylation pathway, converting the amino acid homocysteine to methionine, and serves as the main folate form found in systemic circulation and transported into tissues for physiological use.
SynonymsMethyltetrahydrofolic Acid, Methenyltetrahydrofolic Acid, 5,10-Methenyltetrahydrofolic Acid
Chemical Properties
Molecular Weight455.42
FormulaC20H21N7O6
Cas No.7444-29-3
SmilesO=C1C=2N3[C@@](C[N+](=C3)C4=CC=C(C(N[C@@H](CCC(O)=O)C([O-])=O)=O)C=C4)(CNC2NC(N)=N1)[H]
Relative Density.no data available
Storage & Solubility Information
Storagestore at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Solubility Information
DMSO: 0.8 mg/mL (1.76 mM), Sonication and heating are recommended.
Solution Preparation Table
DMSO
1mg5mg10mg50mg
1 mM2.1958 mL10.9789 mL21.9578 mL109.7888 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
TargetMol | Animal experiments For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.
Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSOTargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSOTargetMol | reagent stock solution to 400 μL PEG300TargetMol | reagent and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH2OTargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.
1 Enter information below:
mg/kg
g
μL
2 Enter the in vivo formulation:
% DMSO
%
% Tween 80
% Saline/PBS/ddH2O

Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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