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AF488 TFP ester TEA salt, 5-isomer (Synonyms: Alexa Fluor 488 TFP ester TEA salt, 5-isomer)

Catalog No. T212879 Copy Product Info
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Alexa Fluor 488 TFP Ester TEA Salt, 5-Isomer is an amine-reactive green fluorescent dye provided as a triethylammonium (TEA) salt. Its tetrafluorophenyl (TFP) ester group reacts specifically with primary amines on proteins, peptides, and other biomolecules to form stable amide bonds. Compared with conventional NHS esters, TFP esters have higher hydrolytic stability in aqueous solutions and remain active under slightly basic conditions for several hours, improving labeling efficiency. This dye is suitable for high-sensitivity fluorescence imaging and biomolecule labeling.

AF488 TFP ester TEA salt, 5-isomer

Copy Product Info
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Catalog No. T212879
Synonyms Alexa Fluor 488 TFP ester TEA salt, 5-isomer

Alexa Fluor 488 TFP Ester TEA Salt, 5-Isomer is an amine-reactive green fluorescent dye provided as a triethylammonium (TEA) salt. Its tetrafluorophenyl (TFP) ester group reacts specifically with primary amines on proteins, peptides, and other biomolecules to form stable amide bonds. Compared with conventional NHS esters, TFP esters have higher hydrolytic stability in aqueous solutions and remain active under slightly basic conditions for several hours, improving labeling efficiency. This dye is suitable for high-sensitivity fluorescence imaging and biomolecule labeling.

AF488 TFP ester TEA salt, 5-isomer
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Product Introduction

Bioactivity
Description
Alexa Fluor 488 TFP Ester TEA Salt, 5-Isomer is an amine-reactive green fluorescent dye provided as a triethylammonium (TEA) salt. Its tetrafluorophenyl (TFP) ester group reacts specifically with primary amines on proteins, peptides, and other biomolecules to form stable amide bonds. Compared with conventional NHS esters, TFP esters have higher hydrolytic stability in aqueous solutions and remain active under slightly basic conditions for several hours, improving labeling efficiency. This dye is suitable for high-sensitivity fluorescence imaging and biomolecule labeling.
SynonymsAlexa Fluor 488 TFP ester TEA salt, 5-isomer
Chemical Properties
Molecular Weight682.53
FormulaC27H14F4N2O11S2
SmilesO=C(O)C=1C=C(C=CC1C=2C=3C=CC(N)=C(C3[O+]=C4C2C=CC(N)=C4S(=O)(=O)O)S(=O)(=O)[O-])C(=O)OC=5C(F)=C(F)C=C(F)C5F
Storage & Solubility Information
StorageKeep away from direct sunlight | Store at -20°C | Shipping with blue ice/Shipping at ambient temperature.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
TargetMol | Animal experiments For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.
Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSOTargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSOTargetMol | reagent stock solution to 400 μL PEG300TargetMol | reagent and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH2OTargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.
1 Enter information below:
mg/kg
g
μL
2 Enter the in vivo formulation:
% DMSO
%
% Tween 80
% Saline/PBS/ddH2O

Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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