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WST-8

Name: WST-8
Brand: TargetMol
SKU: T4018
Price: 33 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T4018Cas No. 193149-74-5

Alias WST8

WST-8 is a water-soluble tetrazolium dye, and WST-8-based assays have higher sensitivity than traditional MTS-based assays.

WST-8

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Purity: 99.93%

Catalog No. T4018Alias WST8Cas No. 193149-74-5

WST-8 is a water-soluble tetrazolium dye, and WST-8-based assays have higher sensitivity than traditional MTS-based assays.

Pack Size	Price	Availability
10 mg	$33	In Stock
25 mg	$50	In Stock
50 mg	$72	In Stock
100 mg	$109	In Stock
500 mg	$328	In Stock

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All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.93%

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COA

Product Introduction

Bioactivity

Description	WST-8 is a water-soluble tetrazolium dye, and WST-8-based assays have higher sensitivity than traditional MTS-based assays.
In vitro	METHODS: A commonly used MTS-based assay was compared to a bioassay using the water-soluble tetrazolium dye WST-8 at a concentration of 7 × 10(5) cells/ml with 800 IU/ml PEGylated G-CSF at 24, 48, 72, and 72 h time points to determine the efficacy of PEGylated G-CSF. In addition, the optimized WST-8 dye-based assay was used to test the potency of various commercially available PEGylated G-CSF formulations. RESULTS The results demonstrated that the WST-8-based assay had greater sensitivity in determining the potency of PEGylated G-CSF using the NFS-60 cell line compared to the conventional MTS-based assay.[1]
Synonyms	WST8

Chemical Properties

Molecular Weight	600.47
Formula	C₂₀H₁₃N₆NaO₁₁S₂
Cas No.	193149-74-5
Smiles	[Na+].COc1cc(ccc1-[n+]1nc(nn1-c1ccc(cc1)[N+]([O-])=O)-c1ccc(cc1S([O-])(=O)=O)S([O-])(=O)=O)[N+]([O-])=O
Relative Density.	no data available
Color	Yellow
Appearance	Solid

Storage & Solubility Information

Storage

keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

H2O: 50 mg/mL (83.13 mM), Sonication is recommended.

DMSO: 255 mg/mL (424.67 mM), Sonication is recommended.

Solution Preparation Table

H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	1.6654 mL	8.3268 mL	16.6536 mL	83.2681 mL
5 mM	0.3331 mL	1.6654 mL	3.3307 mL	16.6536 mL
10 mM	0.1665 mL	0.8327 mL	1.6654 mL	8.3268 mL
20 mM	0.0833 mL	0.4163 mL	0.8327 mL	4.1634 mL
50 mM	0.0333 mL	0.1665 mL	0.3331 mL	1.6654 mL

DMSO

	1mg	5mg	10mg	50mg
100 mM	0.0167 mL	0.0833 mL	0.1665 mL	0.8327 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

WST-8WST 8Inhibitorinhibit