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WST-8

🥰Excellent
Catalog No. T4018Cas No. 193149-74-5
Alias WST8

WST-8 is a water-soluble tetrazolium dye, and WST-8-based assays have higher sensitivity than traditional MTS-based assays.

WST-8

WST-8

🥰Excellent
Purity: 99.93%
Catalog No. T4018Alias WST8Cas No. 193149-74-5
WST-8 is a water-soluble tetrazolium dye, and WST-8-based assays have higher sensitivity than traditional MTS-based assays.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
10 mg$33In StockIn Stock
25 mg$50In StockIn Stock
50 mg$72In StockIn Stock
100 mg$109In StockIn Stock
500 mg$328In StockIn Stock
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
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Batch Information

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Purity:99.93%
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Product Introduction

Bioactivity
Description
WST-8 is a water-soluble tetrazolium dye, and WST-8-based assays have higher sensitivity than traditional MTS-based assays.
In vitro
METHODS: A commonly used MTS-based assay was compared to a bioassay using the water-soluble tetrazolium dye WST-8 at a concentration of 7 × 10(5) cells/ml with 800 IU/ml PEGylated G-CSF at 24, 48, 72, and 72 h time points to determine the efficacy of PEGylated G-CSF. In addition, the optimized WST-8 dye-based assay was used to test the potency of various commercially available PEGylated G-CSF formulations.
RESULTS The results demonstrated that the WST-8-based assay had greater sensitivity in determining the potency of PEGylated G-CSF using the NFS-60 cell line compared to the conventional MTS-based assay.[1]
SynonymsWST8
Chemical Properties
Molecular Weight600.47
FormulaC20H13N6NaO11S2
Cas No.193149-74-5
Smiles[Na+].COc1cc(ccc1-[n+]1nc(nn1-c1ccc(cc1)[N+]([O-])=O)-c1ccc(cc1S([O-])(=O)=O)S([O-])(=O)=O)[N+]([O-])=O
Relative Density.no data available
ColorYellow
AppearanceSolid
Storage & Solubility Information
Storagekeep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Solubility Information
H2O: 50 mg/mL (83.27 mM), Sonication is recommended.
DMSO: 255 mg/mL (424.67 mM), Sonication is recommended.
In Vivo Formulation
10% DMSO+40% PEG300+5% Tween-80+45% Saline: 1 mg/mL (1.67 mM), Sonication is recommeded.
Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.
Solution Preparation Table
H2O/DMSO
1mg5mg10mg50mg
1 mM1.6654 mL8.3268 mL16.6536 mL83.2681 mL
5 mM0.3331 mL1.6654 mL3.3307 mL16.6536 mL
10 mM0.1665 mL0.8327 mL1.6654 mL8.3268 mL
20 mM0.0833 mL0.4163 mL0.8327 mL4.1634 mL
50 mM0.0333 mL0.1665 mL0.3331 mL1.6654 mL
DMSO
1mg5mg10mg50mg
100 mM0.0167 mL0.0833 mL0.1665 mL0.8327 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
TargetMol | Animal experimentsFor example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH2O. So your working solution concentration is 2 mg/mL。
Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSOTargetMol | reagent (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
Preparation method for in vivo formula: Take 50 μL DMSOTargetMol | reagent main solution, add 300 μLPEG300TargetMol | reagent mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH2OTargetMol | reagent mix well and clarify
For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.
All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.
1 Enter information below:
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2 Enter the in vivo formulation:
% DMSO
%
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% Saline/PBS/ddH2O

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

WST-8WST 8Inhibitorinhibit
Related Tags: buy WST-8 | purchase WST-8 | WST-8 cost | order WST-8 | WST-8 chemical structure | WST-8 in vitro | WST-8 formula | WST-8 molecular weight
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