Tween 80

Catalog No. T13947 CAS 9005-65-6

Tween 80 is a nonionic surfactant and emulsifier that reduces bacterial adherence and inhibits biofilm formation. Tween 80 is widely used as a co-solvent in pharmacology experiments because of its ability to enhance the solubility of compounds in the aqueous phase.

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Tween 80, CAS 9005-65-6

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100 mL	In stock	$ 48.00

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Biological Description

Chemical Properties

Storage & Solubility Information

Description	Tween 80 is a nonionic surfactant and emulsifier that reduces bacterial adherence and inhibits biofilm formation. Tween 80 is widely used as a co-solvent in pharmacology experiments because of its ability to enhance the solubility of compounds in the aqueous phase.
In vitro	METHODS: S. aureus, L. monocytogenes, and P. fluorescens were treated with Tween 80 (0.1% (v/v) in TSB), and growth curves were documented by measuring optical densities at OD600 every 20 min. RESULTS: Tween 80 increased the growth rate of S. aureus. tween 80 did not affect the growth of L. monocytogenes. the growth rate of the P. fluorescens tween 80 group was initially higher than that of the control group, but then declined again, with the maximum OD significantly lower than that of the control. [1]
In vivo	METHODS: To study the effect of solvents on locomotor activity in mice, Tween-80/Tween-20 (4%-64% (v/v) in saline) was administered intraperitoneally as a single injection to (BALB/c * DBA/2) F1 mice, and the locomotor activity was monitored for 12 h after the administration. RESULTS: Tween-80 significantly decreased the locomotor activity of mice at a concentration of 32%, while Tween-20 significantly decreased the locomotor activity of mice at a concentration of 16%. [2] METHODS: To detect anti-tumor activity in vivo, ATL (15-30 mg/kg in 5% DMSO + 30% PEG300 + 10% Tween 80 + 55% PBS) was injected intraperitoneally into BALB/c mice harboring human thyroid carcinoma tumor 8505C once daily for nine weeks. RESULTS: ATL inhibited the growth of 8505C cell xenografts in vivo. [3]

Molecular Weight	N/A
CAS No.	9005-65-6

Storage

-20°C for 2 years, 4°C for 1 year

References and Literature

1. Nielsen CK, et al. Effects of Tween 80 on Growth and Biofilm Formation in Laboratory Media. Front Microbiol. 2016 Nov 22;7:1878. 2. Castro CA, et al. Behavioral effects of vehicles: DMSO, ethanol, Tween-20, Tween-80, and emulphor-620. Pharmacol Biochem Behav. 1995 Apr;50(4):521-6. 3. Hu Y, et al. Alantolactone induces concurrent apoptosis and GSDME-dependent pyroptosis of anaplastic thyroid cancer through ROS mitochondria-dependent caspase pathway. Phytomedicine. 2023 Jan;108:154528.

Citations

1. Hu Y, Wen Q, Cai Y, et al. Alantolactone induces concurrent apoptosis and GSDME-dependent pyroptosis of anaplastic thyroid cancer through ROS mitochondria-dependent caspase pathway. Phytomedicine. 2022: 154528. 2. Yang D, Fan Y, Xiong M, et al.Loss of renal tubular G9a benefits acute kidney injury by lowering focal lipid accumulation via CES1.EMBO reports.2023: e56128. 3. Guo Y, Zhu L, Duan Y, et al.Ruxolitinib induces apoptosis and pyroptosis of anaplastic thyroid cancer via the transcriptional inhibition of DRP1-mediated mitochondrial fission.Cell Death & Disease.2024, 15(2): 125.

Dose Conversion

You can also refer to dose conversion for different animals. More

In vivo Formulation Calculator (Clear solution)

Step One: Enter information below

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Step Two: Enter the in vivo formulation

% DMSO+

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% Tween 80+

% ddH₂O

%DMSO+

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Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

Note:

Be sure to add the solvent(s) in order. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

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