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Monochlorobimane

Catalog No. T19028 CAS 76421-73-3

Synonyms: mBBr, Chlorobimane

Monochlorobimane (mBBr) is a fluorescent dye with a λex value of 380 nm and a λem value of 470 nm.Monochlorobimane can be used to measure glutathione (GSH) in cells.

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Monochlorobimane, CAS 76421-73-3

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Biological Description

Chemical Properties

Storage & Solubility Information

Description

Monochlorobimane (mBBr) is a fluorescent dye with a λex value of 380 nm and a λem value of 470 nm.Monochlorobimane can be used to measure glutathione (GSH) in cells.

In vitro

A common technique to measure Glutathione (GSH) in cultured cells is to add Monochlorobimane to the culture medium where it readily enters cells to form a fluorescent GSH-monochlorobimane adduct that can be measured fluorometrically. The Monochlorobimane approach gives comparable results to the HPLC technique. Monochlorobimane concentration can be lowered to 25 mM and still accurately measure homogenate GSH content.[1]

Synonyms	mBBr, Chlorobimane
Molecular Weight	226.66
Formula	C10H11ClN2O2
CAS No.	76421-73-3

Storage

store at low temperature,keep away from direct sunlight

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 45.0 mg/mL (198.5 mM), Sonication is recommended.

References and Literature

1. Kamencic H, et al. Monochlorobimane fluorometric method to measure tissue glutathione. Anal Biochem. 2000 Nov 1;286(1):35-7. 2. Machado M D, et al. Assessment of cellular reduced glutathione content in Pseudokirchneriella subcapitata using monochlorobimane. Journal of applied phycology. 2012; 24: 1509-1516. 3. Hohwy M, et al. Novel prostaglandin D synthase inhibitors generated by fragment-based drug design. J Med Chem. 2008;51(7):2178-2186.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Monochlorobimane 76421-73-3 mBBr Chlorobimane inhibitor inhibit

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