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Methyl-β-cyclodextrin

Catalog No. T4072 CAS 128446-36-6

Synonyms: Methyl-beta-cyclodextrin, beta-Cyclodextrin methyl ethers

Methyl-β-cyclodextrin (Methyl-beta-cyclodextrin) is a macrocyclic compound used as a solubilizer for hydrophobic compounds in biological experiments. Methyl-β-cyclodextrin is also a lipid raft inhibitor with cholesterol-lowering activity and potential antitumor activity.

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Methyl-β-cyclodextrin Chemical Structure

Methyl-β-cyclodextrin, CAS 128446-36-6

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500 mg	In stock	$ 33.00
1 g	In stock	$ 42.00

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Biological Description

Chemical Properties

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Description	Methyl-β-cyclodextrin (Methyl-beta-cyclodextrin) is a macrocyclic compound used as a solubilizer for hydrophobic compounds in biological experiments. Methyl-β-cyclodextrin is also a lipid raft inhibitor with cholesterol-lowering activity and potential antitumor activity.
Targets&IC50	PEL:3.33-4.23 mM.
In vitro	METHODS: Five primary exudative lymphoma (PEL) cell lines, BCBL-1, BC-1, BC-3, TY-1, and GTO, were treated with Methyl-β-cyclodextrin (0-10 mM) for 24 h, and cell viability was measured by MTT assay. RESULTS: Methyl-β-cyclodextrin dose-dependently inhibited the growth of PEL cells with IC50 between 3.33-4.23 mM. [1] METHODS: Chicken hepatocellular carcinoma cells, LMH, were treated with Methyl-β-cyclodextrin (10 mM) for 1 h, then cholesterol (50 µg/mL) was added at different time points before, during, and after infection with FAdV-4, and the expression levels of the target proteins were detected by Western Blot. RESULTS: When LMH cells were pretreated with increasing concentrations of Methyl-β-cyclodextrin prior to infection, penton protein levels decreased in a dose-dependent manner. [2]
In vivo	METHODS: To test the antitumor activity in vivo, Methyl-β-cyclodextrin (500 mg/kg) was intraperitoneally injected into NRJ mice bearing PEL tumor BCBL-1 once a day for twenty-one days. RESULTS: Methyl-β-cyclodextrin significantly inhibited the growth and invasion of PEL cells without significant adverse effects. [1] METHODS: To investigate the modulatory effects on collagen, Methyl-β-cyclodextrin (1.25-5.0 mg/mouse) was intradermally injected into the SKH1 mouse twice a week for two months. RESULTS: Methyl-β-cyclodextrin showed potent COL I up-regulatory activity resulting in increased skin thickness. [3]
Kinase Assay	PEL cells are incubated in triplicate in a 96-well microculture plate in the presence of different concentrations of methyl-β-cyclodextrin (0-10 mM) in a final volume of 0.1 mL for 24 h at 37°C. Subsequently, MTT (0.5 mg/mL final concentration) is added to each well. After 3 h of additional incubation, 100 μL of a 0.04 N HCl is added to dissolve the crystals. Absorption values at 570 nm are determined.
Cell Research	PEL cells are incubated in triplicate in a 96-well microculture plate in the presence of different concentrations of methyl-β-cyclodextrin (0-10 mM) in a final volume of 0.1 mL for 24 h at 37°C. Subsequently, MTT (0.5 mg/mL final concentration) is added to each well. After 3 h of additional incubation, 100 μL of a 0.04 N HCl is added to dissolve the crystals. Absorption values at 570 nm are determined[1].

Synonyms	Methyl-beta-cyclodextrin, beta-Cyclodextrin methyl ethers
Molecular Weight	1310
Formula	C54H94O35
CAS No.	128446-36-6

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 55 mg/mL (41.98 mM)

References and Literature

1. Gotoh K, et al. The antitumor effects of methyl-β-cyclodextrin against primary effusion lymphoma via the depletion of cholesterol from lipid rafts. Biochem Biophys Res Commun. 2014 Dec 12;455(3-4):285-9. 2. Wang T, et al. Fowl adenovirus serotype 4 enters leghorn male hepatocellular cells via the clathrin-mediated endocytosis pathway. Vet Res. 2023 Mar 14;54(1):24. 3. Takeo T, et al. Methyl-beta-cyclodextrin improves fertilizing ability of C57BL/6 mouse sperm after freezing and thawing by facilitating cholesterol efflux from the cells. Biol Reprod. 2008 Mar;78(3):546-51. 4. Zhang Y, Xue W, Zhang W, et al. Histone methyltransferase G9a protects against acute liver injury through GSTP1. Cell Death & Differentiation. 2019: 1-16.

Citations

1. Lu J Y, Huang W T, Zhou K, et al. Microbial Lipopeptide Supramolecular Self‐Assemblies as a Methuosis‐Like Cell Death Inducer with In Vivo Antitumor Activity. Small. 2021: 2104034. 2. Zhang Y, Xue W, Zhang W, et al. Histone methyltransferase G9a protects against acute liver injury through GSTP1. Cell Death & Differentiation. 2020, 27(4): 1243-1258 3. Li X, Tang H, Huang X, et al. Rigidity‐Dependent Placental Cells Uptake of Silk‐Based Microcapsules. Macromolecular bioscience. 2019: 1900105. 4. Zeng L, Liu H, Liu Z, et al.Defected lipid rafts suppress cavin1-dependent IFN-α signaling endosome in paroxysmal nocturnal hemoglobinuria.International Immunopharmacology.2023, 115: 109468. 5. Wang T, Wang L, Li W, et al.Fowl adenovirus serotype 4 enters leghorn male hepatocellular cells via the clathrin-mediated endocytosis pathway.Veterinary Research.2023, 54(1): 1-14.

Related compound libraries

This product is contained In the following compound libraries：

Macrocyclic Compound Library Preclinical Compound Library NO PAINS Compound Library

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Keywords

Methyl-β-cyclodextrin 128446-36-6 Others Inhibitor Methyl-beta-cyclodextrin Methylβcyclodextrin Methyl-b-cyclodextrin beta-Cyclodextrin methyl ethers b-Cyclodextrin methyl ethers Methyl β cyclodextrin β-Cyclodextrin methyl ethers inhibit inhibitor

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