60 kcal% High Fat Diet is a high-fat model feed used for developing animal models of obesity and type II diabetes.

Before conducting formal experiments, it is advised to use preliminary trials to determine the optimal experimental conditions, including animal strain, age, dosage, frequency, duration, as well as measurement time and indicators. A diet consisting of 60 kcal% high fat is effective for developing animal models of obesity and type 2 diabetes (T2DM).

1. Mechanism of Obesity Induction: High-fat diets increase the size and amount of adipose cells, thereby inducing obesity. Modeling specifics for mice: Use MMTV-polyoma middle T antigen (PyMT) transgenic mice at 4 weeks old, administering daily feeding for 8 weeks. Note Tips: Implement a 24-hour fasting method or gradually change diets over 7 days. Feed should be replaced every 2-3 days, discarding the old feed. Opt for sawdust bedding, changing it 1-2 times weekly. Foods with 60% fat have low hardness, so provide chew sticks. Store in a refrigerator. Successful modeling indicators include:
- Body Weight: Mice on a high-fat (HF) diet weigh more than those on a normal diet.
- Histopathology: Shows mild to moderate steatosis.
- Other Indicators: Abdominal visceral fat weight is consistently higher in HF diet mice compared to those on a normal diet.

2. Mechanism of Type 2 Diabetes Induction: A high-fat diet combined with Streptozotocin injection is a common method for developing a T2DM model in rodents and has been used in diabetes-related cognitive impairment studies. Modeling specifics for rats: Use 4-week-old male Sprague-Dawley rats, with daily feeding of a high-fat diet for 4 weeks followed by a single injection of 30 mg/kg Streptozotocin. Note Tips: A 24-hour fasting method or a gradual dietary adjustment over 7 days is suggested. After 6 weeks on a high-fat diet, administer 35 mg/kg STZ intraperitoneally for 3 consecutive days. Successful modeling indicator: Blood glucose level exceeds 300 mg/dL (16.7 mmol/L).