5-TAMRA-SE, an amine-reactive fluorescent agent, forms conjugates that produce bright, pH-insensitive orange-red fluorescence (approximate excitation/emission maxima ~546/579) and exhibit good photostability.

Instructions
1. Dissolving reagent: 5-TAMRA-SE is usually provided in solid form and can be dissolved in anhydrous DMSO or DMF at a recommended concentration of 1-10 mM. It can be diluted to an appropriate concentration as needed.
2. Labeling reaction:
1) Mix the 5-TAMRA-SE solution with the target molecule containing an amine group (such as protein, antibody, etc.). Generally, the pH of the reaction should be kept in the range of 8.0-9.0 to ensure the optimal reactivity of the NHS ester.
2) The reaction time is generally 30 minutes to 1 hour, and the reaction is carried out at room temperature. The labeling reaction can remove unreacted fluorescent dye by centrifugation or remove excess dye by dialysis.
3. Removal of unreacted dye: After the reaction is completed, use methods such as dialysis or gel filtration to remove unreacted fluorescent dye. This step helps to avoid interference from background signals.
4. Storage: The labeled molecules can be stored at 4°C and avoid long-term exposure to strong light to avoid affecting fluorescence stability.