The '40 kcal% Fat, 20 kcal% Fructose and 2% Cholesterol Diet' is a high-fat, high-fructose, and high-cholesterol regimen, designed for developing models of non-alcoholic fatty liver disease (NAFLD) and non-alcoholic steatohepatitis (NASH).

Prior to formal experimentation, it is recommended to determine optimal experimental conditions through preliminary tests, considering factors such as animal strain, age, dosage, frequency, cycle, detection time, and indicators. Consuming a diet high in fat, fructose, and cholesterol over a long period can induce non-alcoholic fatty liver disease (NAFLD) and non-alcoholic steatohepatitis (NASH). To model NAFLD, use male C57BL/6J mice aged 8-11 weeks, administering daily feeding over a 25-week period. Note: Blood samples should be collected after a 5-hour fast at weeks 0 and 25 to evaluate serum biochemical changes. Change feed every 2-3 days, discarding the old feed, and store samples refrigerated. Successful modeling indicators include increased body weight and liver-to-body weight ratio, hepatic steatosis with increased large fat droplets (LDF), along with elevated serum alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), low-density lipoprotein (LDL), triglycerides, total liver cholesterol (TC), free liver cholesterol (FC), and hepatic triglyceride levels.