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3,6-diacetoxy Phthalonitrile

Name: 3,6-diacetoxy Phthalonitrile
Brand: TargetMol
SKU: T36369
Rating: 4.5 (1 reviews)

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Catalog No. T36369Cas No. 83619-73-2

3,6-diacetoxy Phthalonitrile is a fluorescent probe for monitoring intracellular pH by flow cytometry in living cells. It rapidly crosses cell membranes and is cleaved by cytosolic esterases to form the fluorescent pH indicator 2,3-dicyano-hydroquinone (DCH), which is excited at UV wavelengths. DCH can be excited at 351 nm, with pH-dependent emission in the range of 450-476 nm (pH 5.0-10.0) using a potassium-based buffer.

3,6-diacetoxy Phthalonitrile

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Catalog No. T36369Cas No. 83619-73-2

Pack Size	Price	Availability
5 mg	TBD	35 days
10 mg	TBD	35 days
25 mg	TBD	35 days

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Product Introduction

Bioactivity

Description

Chemical Properties

Molecular Weight	244.206
Formula	C₁₂H₈N₂O₄
Cas No.	83619-73-2

Storage & Solubility Information

Storage

keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

Solubility Information

DMSO: 30 mg/mL (122.85 mM), Sonication is recommended.

DMF: 30 mg/mL (122.85 mM), Sonication is recommended.

DMSO:PBS(pH7.2) (1:1): 0.5 mg/mL (2.05 mM), Sonication is recommended.

Solution Preparation Table

DMSO:PBS(pH7.2) (1:1)/DMSO/DMF

	1mg	5mg	10mg	50mg
1 mM	4.0949 mL	20.4745 mL	40.9490 mL	204.7452 mL

DMSO/DMF

	1mg	5mg	10mg	50mg
5 mM	0.8190 mL	4.0949 mL	8.1898 mL	40.9490 mL
10 mM	0.4095 mL	2.0475 mL	4.0949 mL	20.4745 mL
20 mM	0.2047 mL	1.0237 mL	2.0475 mL	10.2373 mL
50 mM	0.0819 mL	0.4095 mL	0.8190 mL	4.0949 mL
100 mM	0.0409 mL	0.2047 mL	0.4095 mL	2.0475 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

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