Notch 1 Protein, Human, Recombinant (His & Avi), Biotinylated

Name: Notch 1 Protein, Human, Recombinant (His & Avi), Biotinylated
Brand: TargetMol
SKU: TMPK-00468
Rating: 4.5 (1 reviews)

(Synonyms: TAN1, Notch-1, NOTCH1, N1, Mis6, lin-12, 9930111A19Rik) Copy Product Info

Synonyms: TAN1, Notch-1, NOTCH1, N1, Mis6, lin-12, 9930111A19Rik

Catalog No. TMPK-00468 Copy Product Info

Notch 1 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 56.5 kDa and the accession number is P46531.

Pack Size	Price	USA Stock	Global Stock
5 µg	$91	7-10 days	7-10 days
10 µg	$148	7-10 days	7-10 days
20 µg	$245	7-10 days	7-10 days
50 µg	$483	7-10 days	7-10 days
100 µg	$813	7-10 days	7-10 days
200 µg	$1,470	7-10 days	7-10 days
500 µg	$3,250	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Human DLL4, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human Notch 1, His Tag with an affinity constant of 0.31 μM as determined in SPR assay.
Description	Notch 1 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 56.5 kDa and the accession number is P46531.
Species	Human
Expression System	HEK293 Cells
Tag	C-His-Avi
Accession Number	P46531
Construction	Ala19-Gln526
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Synonyms	TAN1, Notch-1, NOTCH1, N1, Mis6, lin-12, 9930111A19Rik
Research Background	Human Notch-1 is a 300 kDa type I transmembrane glycoprotein that is one of four human Notch homologues involved in developmental processes.Notch-1 functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.

Chemical Properties

Molecular Weight

56.5 kDa (predicted). Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Recombinant Proteins

Keywords

TAN-1TAN 1NOTCH 1N-1N 1

Related Tags: Notch 1 Protein, Human, Recombinant (His & Avi), Biotinylated chemical structure | Notch 1 Protein, Human, Recombinant (His & Avi), Biotinylated molecular weight