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NKG2D/CD314 Protein, Mouse, Recombinant (His)

Catalog No. TMPJ-00122
Synonyms: KLR, NKG2-D type II integral membrane protein, CD314, NKG2-D-activating NK receptor, KLRK1, CD314 antigen, member 1, NKG2-D, NK cell receptor D, Killer cell lectin-like receptor subfamily K member 1, killer cell lectin-like receptor subfamily K

NKG2D/CD314 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with N-6xHis tag. The predicted molecular weight is 20-30 KDa and the accession number is O54709.

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NKG2D/CD314 Protein, Mouse, Recombinant (His)
Pack Size Availability Price/USD Quantity
10 μg 5 days $ 105.00
50 μg 5 days $ 321.00
500 μg 5 days $ 1,600.00
1 mg 5 days $ 2,400.00
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Biological Description
Technical Params
Product Properties
Description NKG2D/CD314 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with N-6xHis tag. The predicted molecular weight is 20-30 KDa and the accession number is O54709.
Species Mouse
Expression System Human Cells
Tag N-6His
Accession Number O54709
Synonyms KLR, NKG2-D type II integral membrane protein, CD314, NKG2-D-activating NK receptor, KLRK1, CD314 antigen, member 1, NKG2-D, NK cell receptor D, Killer cell lectin-like receptor subfamily K member 1, killer cell lectin-like receptor subfamily K
Amino Acid Phe94
Construction Recombinant Mouse NKG2-D type II Integral Membrane Protein is produced by our Mammalian expression system and the target gene encoding Phe94 - Val232 is expressed with a 6His tag at the N-terminus.
Protein Purity Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Molecular Weight 20-30 KDa, reducing conditions
Endotoxin Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Formulation Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Reconstitution Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 
Stability & Storage

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

Research Background NKG2-D type II integral membrane protein (NKG2D) is a type II transmembrane glycoprotein which belongs to the CD94/NKG2 family. NKG2D is expressed on natural killer (NK) cells, CD8+ alpha-beta and gamma-delta T-cells. As an activating and costimulatory receptor, it involved in immunosurveillance upon binding to various cellular stress-inducible ligands displayed at the surface of autologous tumor cells and virus-infected cells. It provides both stimulatory and costimulatory innate immune responses on activated killer (NK) cells, leading to cytotoxic activity. It stimulates perforin-mediated elimination of ligand-expressing tumor cells. Signaling involves calcium influx, culminating in the expression of TNF-alpha. NKG2D participates in NK cell-mediated bone marrow graft rejection and survival of NK cells. It Binds to ligands belonging to various subfamilies of MHC class I-related glycoproteins including MICA, MICB, RAET1E, RAET1G, ULBP1, ULBP2, ULBP3 (ULBP2>ULBP1>ULBP3) and ULBP4.

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Keywords

NKG2D/CD314 Protein, Mouse, Recombinant (His) KLR KLRK 1 NKG2-D type II integral membrane protein CD314 CD-314 NKG2-D-activating NK receptor KLRK1 CD 314 CD314 antigen member 1 KLRK-1 NKG2-D NK cell receptor D Killer cell lectin-like receptor subfamily K member 1 killer cell lectin-like receptor subfamily K recombinant recombinant-proteins proteins protein

 

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