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Leptin Protein, Carassius auratus, Recombinant (His)

(Synonyms: OBS, Obesity Factor, Obese Protein, OB, Leptin, LEP) Copy Product Info

Synonyms: OBS, Obesity Factor, Obese Protein, OB, Leptin, LEP

Catalog No. TMPJ-00606 Copy Product Info
Leptin is a hormone secreted from white adipocytes and plays important role in the regulation of food intake and energy balance. Leptin functions via signaling pathways involving OB-R in hypothalamus. In mammals, leptin is mainly produced by the adipose tissue and encodes body fat reserves, acting as a short-term satiety signal. In fish, the presence of a leptin-like peptide was first evidenced by immuno-cross-reactivity, and its existence was certainly demonstrated after the finding by synteny of a leptin sequence in the pufferfish.
Pack SizePriceUSA StockGlobal StockQuantity
5 μg$807-10 days7-10 days
10 μg$1297-10 days7-10 days
20 μg$1987-10 days7-10 days
50 μg$3907-10 days7-10 days
100 μg$6267-10 days7-10 days
200 μg$9877-10 days7-10 days
500 μg$1,9007-10 days7-10 days
1 mg$2,7307-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Bioactivity
Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description
Leptin is a hormone secreted from white adipocytes and plays important role in the regulation of food intake and energy balance. Leptin functions via signaling pathways involving OB-R in hypothalamus. In mammals, leptin is mainly produced by the adipose tissue and encodes body fat reserves, acting as a short-term satiety signal. In fish, the presence of a leptin-like peptide was first evidenced by immuno-cross-reactivity, and its existence was certainly demonstrated after the finding by synteny of a leptin sequence in the pufferfish.
Species
Carassius auratus
Expression System
P. pastoris (Yeast)
TagN-8xHis
Accession NumberB8YI02
Amino AcidPro22-Cys171
ConstructionPro22-Cys171
Protein Purity
Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Endotoxin< 0.1 ng/µg (1 EU/µg) as determined by LAL test.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Citrate, 8% Trehalose, 4% Mannitol, 0.02% Tween 80 (w/v), pH 5.5.
ReconstitutionReconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & StorageLyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
SynonymsOBS, Obesity Factor, Obese Protein, OB, Leptin, LEP
Research Background
Chemical Properties
Molecular Weight17 KDa (reducing condition)
Storage & Solubility Information
StorageLyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Dose Conversion

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Related Tags: Leptin Protein, Carassius auratus, Recombinant (His) chemical structure | Leptin Protein, Carassius auratus, Recombinant (His) molecular weight