ISG15 Protein, Human, Recombinant (mature form)

Name: ISG15 Protein, Human, Recombinant (mature form)
Brand: TargetMol
SKU: TMPY-02467
Rating: 4.5 (1 reviews)

(Synonyms: UCRP, ISG15 ubiquitin-like modifier, IP17, IMD38, IFI15, hUCRP, G1P2) Copy Product Info

Synonyms: UCRP, ISG15 ubiquitin-like modifier, IP17, IMD38, IFI15, hUCRP, G1P2

Catalog No. TMPY-02467 Copy Product Info

ISG15 Protein, Human, Recombinant (mature form) is expressed in E. coli expression system. The predicted molecular weight is 17.2 kDa and the accession number is AAH09507.1.

Pack Size	Price	USA Stock	Global Stock
50 µg	$38	7-10 days	7-10 days
100 µg	$58	7-10 days	7-10 days
200 µg	$93	7-10 days	7-10 days
500 µg	$172	7-10 days	7-10 days
1 mg	$343	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Batch Information

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Purity:≥ 95 % as determined by SDS-PAGE. ≥ 85 % as determined by SEC-HPLC.

Resource Download

COA User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	ISG15 Protein, Human, Recombinant (mature form) is expressed in E. coli expression system. The predicted molecular weight is 17.2 kDa and the accession number is AAH09507.1.
Species	Human
Expression System	E. coli
Tag	Tag Free
Accession Number	AAH09507.1
Construction	A DNA sequence encoding the mature form of human ISG15 (AAH09507.1) (Met 1-Gly 157) was expressed and purified. Predicted N terminal: Met 1
Protein Purity	> 95 % as determined by SDS-PAGE
Endotoxin	Please contact us for more information.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 50 mM Tris, pH 8.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	UCRP, ISG15 ubiquitin-like modifier, IP17, IMD38, IFI15, hUCRP, G1P2
Research Background	Interferon-induced 17 kDa protein (ISG15), a 15-kDa protein of unique primary amino acid sequence, functions intracellularly as a ubiquitin homolog and a cytokine that induces production of IFN-gamma and augments NK / lymphokine-activated killer cell proliferation and function. ISG15 is secreted from monocytes and lymphocytes. ISG15 is a ubiquitin-like molecule that is strongly upregulated by type I interferons as a primary response to diverse microbial and cellular stress stimuli. Alterations in the ISG15 signaling pathway have also been found in several human tumor entities. In addition to being stimulated by type I interferon, expression of ISG15 is greatly induced by viral or bacterial infection through the Janus kinase/signal transducer and activator of transcription (Jak / STAT) signaling pathway. After induction, ISG15 is secreted by monocytes, B- and T-lymphocytes, and fibroblasts. We demonstrate the novel way in which the function of the ISG15 protein is inhibited by influenza B virus, which strongly induces the ISG15 protein: a specific region of the influenza B virus NS1 protein, which includes part of its effector domain, blocks the covalent linkage of ISG15 to its target proteins both in vitro and in infected cells.

Chemical Properties

Molecular Weight

17.2 kDa (predicted); 15 kDa (reducing conditions)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.