IRE1 Protein, Human, Recombinant (aa 465-977, His & GST)

Catalog No. TMPY-04413

IRE1 Protein, Human, Recombinant (aa 465-977, His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 86 kDa and the accession number is O75460-1.

IRE1 Protein, Human, Recombinant (aa 465-977, His & GST)

Catalog No. TMPY-04413

IRE1 Protein, Human, Recombinant (aa 465-977, His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 86 kDa and the accession number is O75460-1.

Pack Size	Price	Availability
50 μg	$320	In Stock
100 μg	$547	7-10 days
200 μg	$935	7-10 days
500 μg	$1,890	7-10 days

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Product Information

Biological Activity	The specific activity was determined to be 5 nmol/min/mg using MBP as substrate
Description	IRE1 Protein, Human, Recombinant (aa 465-977, His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 86 kDa and the accession number is O75460-1.
Species	Human
Expression System	Baculovirus Insect Cells
Tag	His, GST
Accession Number	O75460-1
Synonyms	IRE1P,IRE1a,IRE1,hIRE1p,endoplasmic reticulum to nucleus signaling 1
Construction	A DNA sequence encoding the human ERN1 isoform 1 (O75460-1) cytoplasmic domain (Pro 465-Leu 977) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus. Predicted N terminal: Met
Protein Purity	> 80 % as determined by SDS-PAGE
Molecular Weight	86 kDa (predicted); 90 kDa (reducing conditions)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Supplied as sterile 20 mM Tris, 500M NaCl, 25% glycerol, 0.1 mM EDTA, 0.5 mM TCEP, 2 mM GSH, pH 7.5.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store the product under sterile conditions at -20°C to -80°C. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	Shipping with blue ice.
Research Background	Endoplasmic reticulum stress and hypoxia are necessary components of malignant tumors growth and suppression of ERN1 (from endoplasmic reticulum to nuclei-1) signalling pathway, which is linked to the apoptosis and cell death processes, significantly decreases proliferative processes. An enhanced expression of TP53 gene in ERN1 knockdown glioma cells correlates with the decreased level of ubiquitin ligase MDM2 and increased expression level of USP7 which deubiquitinates TP53 and MDM2 and induces TP53-dependent cell growth repression and apoptosis. Thus, the expression of genes encoding TP53 and related to TP53 factors depends upon the endoplasmic reticulum stress signaling as well as on hypoxia, and correlates with suppression of glioma growth under ERN1 knockdown. The dependence of insulin-like growth binding proteins as well as IGF2BP3 and HTRA1 gene expressions in U87 glioma cells on ERN1 signaling enzyme function and hypoxia, indicating its participation in the regulation of metabolic and proliferative processes via IGF/INS receptors, because endoplasmic reticulum stress is an important component of tumor growth and metabolic diseases.