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CD122/IL2RB Protein, Human, Recombinant (His & Avi)

(Synonyms: RP5-1170K4.6, p75, P70-75, IL2RB, IL-2R subunit β, IL-2R subunit beta, IL-2 R β/CD122, IL-2 R β, IL-2 R beta, IL-15 R β, IL-15 R beta, CD122) Copy Product Info

Synonyms: RP5-1170K4.6, p75, P70-75, IL2RB, IL-2R subunit β, IL-2R subunit beta, IL-2 R β/CD122, IL-2 R β, IL-2 R beta, IL-15 R β, IL-15 R beta, CD122

Catalog No. TMPK-00320 Copy Product Info
IL-2 R beta/CD122 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 27.5 kDa and the accession number is P14784.

Batch Information

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Purity:> 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Appearance:Lyophilized powder
Color:White
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Product Introduction

Bioactivity
Bioactivity
Human IL-2 R beta, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-2, No Tag with an affinity constant of 0.12 μM as determined in SPR assay.
Description
IL-2 R beta/CD122 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 27.5 kDa and the accession number is P14784.
Species
Human
Expression System
HEK293 Cells
TagC-His-Avi
Accession NumberP14784
ConstructionAla27-Asp239
Protein Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
ReconstitutionReconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
SynonymsRP5-1170K4.6, p75, P70-75, IL2RB, IL-2R subunit β, IL-2R subunit beta, IL-2 R β/CD122, IL-2 R β, IL-2 R beta, IL-15 R β, IL-15 R beta, CD122
Research Background
IL-2 R beta is a member of the cytokine receptor superfamily. Human IL-2 R beta cDNA encodes a 551 amino acid residue precursor Type I membrane protein with a 26 residue signal peptide, a 214 residue extracellular region, a 25 residue transmembrane region and a 286 residue cytoplasmic domain. Functional IL-2 receptors can exist in two affinity states on cell surfaces, the high affinity complex consisting of heterotrimers of the alpha, beta, and gamma chains, and the intermediate affinity complex comprising heterodimers of the beta and gamma chains.
Chemical Properties
Molecular Weight27.5 kDa (predicted). Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Keywords

Related Tags: CD122/IL2RB Protein, Human, Recombinant (His & Avi) chemical structure | CD122/IL2RB Protein, Human, Recombinant (His & Avi) molecular weight