IL-15 & IL-15RA Protein, Mouse, Recombinant (His)

Catalog No. TMPY-06531

IL-15 & IL-15RA Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 34.46 kDa and the accession number is P48346-1 & Q60819-1.

IL-15 & IL-15RA Protein, Mouse, Recombinant (His)

Catalog No. TMPY-06531

IL-15 & IL-15RA Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 34.46 kDa and the accession number is P48346-1 & Q60819-1.

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100 μg	$700	In Stock
1 mg	$4,560	7-10 days

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Product Information

Biological Activity	Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	IL-15 & IL-15RA Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 34.46 kDa and the accession number is P48346-1 & Q60819-1.
Species	Mouse
Expression System	HEK293 Cells
Tag	C-His
Accession Number	P48346-1 & Q60819-1
Construction	A DNA sequence encoding the mouse IL15 (P48346-1)(Met1-Ser162) was expressed with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the mouse IL15RA (Q60819-1) (Met1-Lys205) was expressed with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the IL15 & IL15RA heterodimer was purified. Predicted N terminal: Asn 49 & Gly 33
Protein Purity	> 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.
Molecular Weight	34.46 kDa (predicted); 50.02, 23.49, 21.78 and 19.76 kDa (reducing conditions)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	IL-15 and IL-15 receptor alpha (IL-15Rα) play a significant role in multiple aspects of T cell biology. Interleukin (IL)-15 is essential for natural killer (NK), NKT and memory (m) CD8+ T cell development and function, and is currently under investigation as an immunotherapeutic agent for the treatment of cancer. Recently, the creation of IL-15 superagonist by complexing IL-15 and its high affinity receptor alpha (IL-15 Rα) in solution, inspired by the natural trans-presentation of IL-15, advances the potential of IL-15-based tumor immunotherapy. Gene therapy using engineered cells co-expressing IL-15/IL-15 Rα complex for cancer treatment is also emerging.