IkB alpha/NFKBIA Protein, Human, Recombinant (His)

Catalog No. TMPY-01710

Synonyms: IkB α, IKBA, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, α, NFKBI, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha, MAD-3, nuclear factor of κ light polypeptide gene enhancer in B-cells inhibitor, alpha

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (IkB alpha, NFKBIA, or IKBA), is a member of the NF-kappa-B inhibitor family that function to inhibit the NF-kB transcription factor. NFKBIA inhibits NF-kB by masking the nuclear localization signals (NLS) of NF-kB proteins and keeping them sequestered in an inactive state in the cytoplasm. Also, NFKBIA blocks the ability of NF-κB transcription factors to bind to DNA, which is required for NF-kB's proper functioning. Signal-induced degradation of I kappa B alpha exposes the nuclear localization signal of NF-kappa B, thus allowing it to translocate into the nucleus and activate transcription from responsive genes. An autoregulatory loop is established when NF-kappa B induces expression of the I kappa B alpha gene and newly synthesized I kappa B alpha accumulates in the nucleus where it negatively regulates NF-kappa B-dependent transcription. As part of this post-induction repression, the nuclear export signal on I kappa B alpha mediates the transport of NF-kappa B-I kappa B alpha complexes from the nucleus to the cytoplasm. Deletion of NFKBIA has an effect that is similar to the effect of EGFR amplification in the pathogenesis of glioblastoma and is associated with comparatively short survival. Polymorphisms in NFKBIA may be important in pre-disposition to and outcome after treatment, of multiple myeloma (MM). The NFKBIA gene product, IkappaBalpha, binds to NF-kappaB preventing its activation and is important in mediating resistance to apoptosis in B-cell lymphoproliferative diseases.

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IkB alpha/NFKBIA Protein, Human, Recombinant (His)

Pack Size	Availability	Price/USD	Quantity
50 μg	5 days	$ 600.00

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Biological Description

Technical Params

Product Properties

References and Literature

Description

Species	Human
Expression System	E. coli
Tag	His
Accession Number	P25963
Synonyms	IkB α, IKBA, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, α, NFKBI, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha, MAD-3, nuclear factor of κ light polypeptide gene enhancer in B-cells inhibitor, alpha
Construction	A DNA sequence encoding the human NFKBIA (NP_065390.1) (Phe 2-Leu 317) was expressed, with a polyhistide tag at the N-terminus.
Protein Purity	> 90 % as determined by SDS-PAGE
Molecular Weight	Approxiamtely 36.4 kDa

Endotoxin	Please contact us for more information.
Formulation	Lyophilized from sterile 50mM Tris, 0. 5M NaCl, pH 8.0. Pleasecon tact usfor any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (IkB alpha, NFKBIA, or IKBA), is a member of the NF-kappa-B inhibitor family that function to inhibit the NF-kB transcription factor. NFKBIA inhibits NF-kB by masking the nuclear localization signals (NLS) of NF-kB proteins and keeping them sequestered in an inactive state in the cytoplasm. Also, NFKBIA blocks the ability of NF-κB transcription factors to bind to DNA, which is required for NF-kB's proper functioning. Signal-induced degradation of I kappa B alpha exposes the nuclear localization signal of NF-kappa B, thus allowing it to translocate into the nucleus and activate transcription from responsive genes. An autoregulatory loop is established when NF-kappa B induces expression of the I kappa B alpha gene and newly synthesized I kappa B alpha accumulates in the nucleus where it negatively regulates NF-kappa B-dependent transcription. As part of this post-induction repression, the nuclear export signal on I kappa B alpha mediates the transport of NF-kappa B-I kappa B alpha complexes from the nucleus to the cytoplasm. Deletion of NFKBIA has an effect that is similar to the effect of EGFR amplification in the pathogenesis of glioblastoma and is associated with comparatively short survival. Polymorphisms in NFKBIA may be important in pre-disposition to and outcome after treatment, of multiple myeloma (MM). The NFKBIA gene product, IkappaBalpha, binds to NF-kappaB preventing its activation and is important in mediating resistance to apoptosis in B-cell lymphoproliferative diseases.

References and Literature

1. Verma IM,et al.(1995) Rel/NF-kappa B/I kappa B family: intimate tales of association and dissociation. Genes Dev. 9 (22): 2723-35. 2. Jacobs MD,et al.(1998) Structure of an IkappaBalpha/NF-kappaB complex. Cell 95 (6): 749-58.Hay RT,et al.(1999) Control of NF-kappa B transcriptional activation by signal induced proteolysis of I kappa B alpha. Philos Trans R Soc Lond B Biol Sci. 354(1389): 1601-9. 3. Spink CF,et al.(2007) Haplotypic structure across the I kappa B alpha gene (NFKBIA) and association with multiple myeloma. Cancer Lett. 246(1-2): 92-9. 4. Bredel M,et al.(2011) NFKBIA deletion in glioblastomas. N Engl J Med. 364(7): 627-37.

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Keywords

IkB alpha/NFKBIA Protein, Human, Recombinant (His) IkB α IKBA MAD3 nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, α NFKBI nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha MAD-3 nuclear factor of κ light polypeptide gene enhancer in B-cells inhibitor, alpha MAD 3 recombinant recombinant-proteins proteins protein