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SMAD3 Protein, Human, Recombinant (His & Flag)

Catalog No. TMPJ-00271
Synonyms: SMAD 3, Mothers against DPP homolog 3, hMAD-3, MADH3, JV15-2, SMAD family member 3, SMAD3, MAD homolog 3, Mothers against decapentaplegic homolog 3, hSMAD3, Mad3

Mothers against decapentaplegic homolog 3(SMAD3) is a cytoplasm protein which belongs to the dwarfin/SMAD family. Smad proteins undergo rapid nuclear translocation upon stimulation by transforming growth factor and in so doing transduce the signal into the nucleus. Receptor-regulated SMAD is an intracellular signal transducer and transcriptional modulator activated by TGF-beta and activin type 1 receptor kinases. SMAD3 binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. It also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. SMAD3 has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive.

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SMAD3 Protein, Human, Recombinant (His & Flag)
Pack Size Availability Price/USD Quantity
10 μg 5 days $ 110.00
50 μg 5 days $ 310.00
500 μg 5 days $ 1,390.00
1 mg 5 days $ 1,980.00
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Biological Description
Technical Params
Product Properties
Description Mothers against decapentaplegic homolog 3(SMAD3) is a cytoplasm protein which belongs to the dwarfin/SMAD family. Smad proteins undergo rapid nuclear translocation upon stimulation by transforming growth factor and in so doing transduce the signal into the nucleus. Receptor-regulated SMAD is an intracellular signal transducer and transcriptional modulator activated by TGF-beta and activin type 1 receptor kinases. SMAD3 binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. It also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. SMAD3 has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive.
Species Human
Expression System E. coli
Tag N-6His-Flag
Accession Number P84022
Synonyms SMAD 3, Mothers against DPP homolog 3, hMAD-3, MADH3, JV15-2, SMAD family member 3, SMAD3, MAD homolog 3, Mothers against decapentaplegic homolog 3, hSMAD3, Mad3
Amino Acid Ser2-Ser425
Construction Recombinant Human Mothers Against Decapentaplegic Homolog 3 is produced by our E.coli expression system and the target gene encoding Ser2-Ser425 is expressed with a 6His, Flag tag at the N-terminus.
Protein Purity Greater than 85% as determined by reducing SDS-PAGE. (QC verified)
Molecular Weight 50-60 KDa, reducing conditions
Endotoxin Less than 1 EU/µg as determined by LAL test.
Formulation Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 500mM NaCl, 10% Glycerol, 2mM EDTA, pH 8.0.
Stability & Storage

Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.

Shipping

The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.

Research Background Mothers against decapentaplegic homolog 3(SMAD3) is a cytoplasm protein which belongs to the dwarfin/SMAD family. Smad proteins undergo rapid nuclear translocation upon stimulation by transforming growth factor and in so doing transduce the signal into the nucleus. Receptor-regulated SMAD is an intracellular signal transducer and transcriptional modulator activated by TGF-beta and activin type 1 receptor kinases. SMAD3 binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. It also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. SMAD3 has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive.

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Keywords

SMAD3 Protein, Human, Recombinant (His & Flag) SMAD 3 Mothers against DPP homolog 3 SMAD-3 hMAD-3 MADH3 JV15-2 SMAD family member 3 SMAD3 MADH-3 MAD homolog 3 Mothers against decapentaplegic homolog 3 hSMAD3 Mad3 MADH 3 recombinant recombinant-proteins proteins protein

 

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