CD3E & CD3G Heterodimer Protein, Human, Recombinant (Fc-Flag & Fc-His) is expressed in HEK293 with Fc-Flag and Fc-His tag. The predicted molecular weight is 78.36 kDa. Accession number: NP_000724.1
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
50 μg | 5 days | $ 700.00 | |
500 μg | 5 days | $ 4,350.00 |
Description | CD3E & CD3G Heterodimer Protein, Human, Recombinant (Fc-Flag & Fc-His) is expressed in HEK293 with Fc-Flag and Fc-His tag. The predicted molecular weight is 78.36 kDa. Accession number: NP_000724.1 |
Species | Human |
Expression System | HEK293 |
Tag | Fc-Flag,Fc-His |
Accession Number | NP_000724.1 |
Synonyms | CD3e molecule, epsilon (CD3-TCR complex), TCRE, CD3e molecule, ε (CD3-TCR complex), T3E, IMD18, CD3E & CD3G Heterodimer |
Construction | A DNA sequence encoding the (Met1-Asp126) of human CD3E (NP_000724.1) was fused with a C-terminal Fc region of human IgG1 tag followed by a FLAG tag, constructed the plasmid 1; A DNA sequence encoding the (Met1-Ser116) of human CD3G (NP_000064.1) was fused with a C-terminal Fc region of human IgG1 tag followed by a polyhistidine tag, constructed the plasmid 2. The two plasmids were co-expressed and the Human CD3E/CD3G heterodimer was purified. |
Protein Purity | ≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC. |
Molecular Weight | 78.36 kDa (predicted) |
Endotoxin | < 1.0 EU per μg protein as determined by the LAL method. |
Formulation | Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA. |
Reconstitution | A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information. |
Stability & Storage |
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Shipping |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
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Please read the User Guide of Recombinant Proteins for more specific information.
CD3E & CD3G Heterodimer Protein, Human, Recombinant (Fc-Flag & Fc-His) IMD-18 CD3e molecule, epsilon (CD3-TCR complex) CD3-TCR complex TCRE CD3e molecule, ε (CD3-TCR complex) T3E IMD 18 IMD18 CD3e molecule, epsilon CD3E & CD3G Heterodimer recombinant recombinant-proteins proteins protein