CD39 Protein, Human, Recombinant (His)

CD39, also known as ENTPD1, belongs to the GDA1/CD39 NTPase family. It is expressed primarily on activated lymphoid cells and can also be detected in endothelial tissues. The vascular isoform and the placental isoform II are present in both placenta and umbilical vein, whereas placental isoform I is present in placenta only. CD39 can hydrolyze both nucleoside triphosphates and diphosphates. It is the dominant ecto nucleotidase of vascular and placental trophoblastic tissues and appears to modulate the functional expression of type 2 purinergic (P2) G protein coupled receptors (GPCRs). CD39 transgenic mice exhibit impaired platelet aggregation, prolonged bleeding times, and resistance to systemic thromboembolism. There is a correlation between ATP hydrolysis and triglycerides in patients with chronic heart disease, suggesting a relationship between ATP diphosphohydrolase and thrombogenesis. In the nervous system, CD39 could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission.

Pack Size	Availability	Price/USD
50 μg	In stock	$ 255.00
100 μg	5 days	$ 451.00
200 μg	5 days	$ 797.00
500 μg	5 days	$ 1,690.00

Pack Size

Availability

Price/USD

Quantity

50 μg

In stock

$ 255.00

100 μg

5 days

$ 451.00

200 μg

5 days

$ 797.00

500 μg

5 days

$ 1,690.00

Biological Information

Measured by its ability to hydrolyze the 5’-phosphate groups from the substrate adenosine-5’-triphosphate (ATP). The specific activity is >5000 pmol/min/µg.

Description

Species	Human
Expression System	Baculovirus-Insect Cells
Tag	His
Accession Number	P49961-1
Synonyms	SPG64, CD39, ectonucleoside triphosphate diphosphohydrolase 1, NTPDase-1, ATPDase
Construction	A DNA sequence encoding the human ENTPD1 (NP_001767.3) (Thr38-Val478) was expressed with a polyhistidine tag at the C-terminus.
Protein Purity	≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
Molecular Weight	Approxiamtely 51.8 kDa

Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method.
Formulation	Supplied as sterile 20mM Tris, 300mM NaCl, 10% Glycerol, pH 8.0. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	Solution. It is shipped out with blue ice.
Research Background	CD39, also known as ENTPD1, belongs to the GDA1/CD39 NTPase family. It is expressed primarily on activated lymphoid cells and can also be detected in endothelial tissues. The vascular isoform and the placental isoform II are present in both placenta and umbilical vein, whereas placental isoform I is present in placenta only. CD39 can hydrolyze both nucleoside triphosphates and diphosphates. It is the dominant ecto nucleotidase of vascular and placental trophoblastic tissues and appears to modulate the functional expression of type 2 purinergic (P2) G protein coupled receptors (GPCRs). CD39 transgenic mice exhibit impaired platelet aggregation, prolonged bleeding times, and resistance to systemic thromboembolism. There is a correlation between ATP hydrolysis and triglycerides in patients with chronic heart disease, suggesting a relationship between ATP diphosphohydrolase and thrombogenesis. In the nervous system, CD39 could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission.