BAFF/TNFSF13B Protein, Mouse, Recombinant (mFc)

Catalog No. TMPJ-01391

Synonyms: TALL-1, Dendritic cell-derived TNF-like molecule, BLyS, Tumor necrosis factor ligand superfamily member 13B, BAFF, B lymphocyte stimulator, B-cell-activating factor, TNF- and APOL-related leukocyte expressed ligand 1

TNFSF13B/TNFSF20 belongs to the tumor necrosis factor family. It abundantly is expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas. Isoform 2 is expressed in many myeloid cell lines. Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response. Isoform 2 seems to inhibit isoform 1 secretion and bioactivity. Isoform 3 acts as a transcription factor for its own parent gene, in association with NF-kappa-B p50 subunit, at least in autoimmune and proliferative B-cell diseases. The presence of Delta4BAFF is essential for soluble BAFF release by IFNG/IFN-gamma-stimulated monocytes and for B-cell survival. It can directly or indirectly regulate the differential expression of a large number of genes involved in the innate immune response and the regulation of apoptosis. Isoform 2 heteromultimerizes with isoform 1, probably limiting the amount of functional isoform 1 on the cell surface. Isoform 3 is unlikely form trimers or bind to BAFF receptors. Mature human BAFF consists of a 46 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 218 aa extracellular domain (ECD) with a stalk region and one TNF-like domain. Within aa 134-285 of the ECD, human BAFF shares 72% aa sequence identity with mouse BAFF.

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BAFF/TNFSF13B Protein, Mouse, Recombinant (mFc)

Pack Size	Availability	Price/USD
10 μg	In stock	$ 56.00
50 μg	5 days	$ 169.00
500 μg	5 days	$ 1,380.00
1 mg	5 days	$ 3,220.00

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Biological Description

Technical Params

Product Properties

Biological Information

Loaded Human BCMA-His on HIS1K Biosensor, can bind Mouse BAFF-mFc with an affinity constant of 1.58 nM as determined in BLI assay. (Regularly tested)

Description

Species	Mouse
Expression System	Human Cells
Tag	N-mFc
Accession Number	Q9WU72
Synonyms	TALL-1, Dendritic cell-derived TNF-like molecule, BLyS, Tumor necrosis factor ligand superfamily member 13B, BAFF, B lymphocyte stimulator, B-cell-activating factor, TNF- and APOL-related leukocyte expressed ligand 1
Amino Acid	Ala127-Leu309
Construction	Recombinant Mouse TNF Ligand Superfamily Member 13B is produced by our Mammalian expression system and the target gene encoding Ala127-Leu309 is expressed with a mFc tag at the N-terminus.
Protein Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Molecular Weight	45-60 KDa, reducing conditions

Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Formulation	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Stability & Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Research Background	TNFSF13B/TNFSF20 belongs to the tumor necrosis factor family. It abundantly is expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas. Isoform 2 is expressed in many myeloid cell lines. Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response. Isoform 2 seems to inhibit isoform 1 secretion and bioactivity. Isoform 3 acts as a transcription factor for its own parent gene, in association with NF-kappa-B p50 subunit, at least in autoimmune and proliferative B-cell diseases. The presence of Delta4BAFF is essential for soluble BAFF release by IFNG/IFN-gamma-stimulated monocytes and for B-cell survival. It can directly or indirectly regulate the differential expression of a large number of genes involved in the innate immune response and the regulation of apoptosis. Isoform 2 heteromultimerizes with isoform 1, probably limiting the amount of functional isoform 1 on the cell surface. Isoform 3 is unlikely form trimers or bind to BAFF receptors. Mature human BAFF consists of a 46 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 218 aa extracellular domain (ECD) with a stalk region and one TNF-like domain. Within aa 134-285 of the ECD, human BAFF shares 72% aa sequence identity with mouse BAFF.

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Keywords

BAFF/TNFSF13B Protein, Mouse, Recombinant (mFc) TALL-1 Dendritic cell-derived TNF-like molecule BLyS TALL 1 Tumor necrosis factor ligand superfamily member 13B TALL1 BAFF B lymphocyte stimulator B-cell-activating factor TNF- and APOL-related leukocyte expressed ligand 1 recombinant recombinant-proteins proteins protein