Insulin-Transferrin-Selenium Supplement (100×)

• C0005 Cell Counting Kit-8 (CCK-8)

• C0199 Serum/Protein-Free Cell Freezing Medium

• C0201 0.25% Trypsin-EDTA, phenol red (1×)

• C0051 Penicillin-Streptomycin Solution (100×), Sterile

Suitable for general serum-free or low-serum cell culture systems to support the growth and maintenance of a variety of routine cell types.

I. Preparation:

Ensure the product is fully thawed and mixed thoroughly before use. Perform all procedures under sterile conditions to prevent contamination.

II. Addition Ratio:

Add the supplement to culture medium at a 1:100 ratio (e.g., 1 mL supplement per 100 mL complete culture medium). Mix gently to ensure uniform distribution.

III. Instructions:

After addition, the medium can be used directly for cell culture without further treatment. Depending on the specific cell type and experimental requirements, the serum concentration may be adjusted, or the product may be used in combination with low-serum or serum-free culture systems. During the initial stage of culture, it is recommended to supplement the medium with 2%–4% fetal bovine serum (FBS). Once the cells have gradually adapted to the culture conditions, the FBS concentration may be further reduced.

Store at 4°C protected from light. Stable for one year.

1.Practice sterile techniques to prevent microbial contamination.

2.Some cells may exhibit reduced growth rates immediately after lowering serum concentration. It is recommended to gradually reduce serum levels to allow for adaptation.

3.This product is for professional research use only. It must not be used for clinical diagnosis, treatment, food, or pharmaceutical applications, and should not be stored in residential or other non-professional settings.

4.This product may be irritating to the skin, eyes, and respiratory tract. To ensure safety, always wear a lab coat and disposable gloves when handling.

• C0213-Instruction Manual(318 KB)