TSQ is a zinc fluorescent probe and zinc fluorescent chelator that binds to zinc to form a complex that induces apoptosis and can be used for imaging zinc proteins (λmax ~470 nm.) Light emitted by TSQ can be quenched by the Zn(2+) chelator, TPEN.

Instructions:
a. Reagent preparation: Prepare TSQ fluorescent probe solution according to the experimental purpose.
b. Operation steps:
1. Culture the cells to be tested to an appropriate density and wash the cells with PBS buffer.
2. Add an appropriate amount of TSQ fluorescent probe solution to a final concentration that depends on the cell type and experimental requirements, generally 1-10 μM.
3. Incubate in a 37°C, 5% CO₂ incubator for 15-60 minutes.
4. After incubation, wash the cells with PBS buffer to remove unbound TSQ probes.
5. Observe the cells under a fluorescence microscope, set the excitation wavelength near the maximum excitation wavelength of TSQ, such as around 470 nm, and detect the fluorescence signal of zinc ions in the cells.