Rhodamine 101 chloride is a fluorescent dye with excitation/emission wavelengths of 565/595 nm, serving as a reference standard for fluorescence quantum yield measurements.

Usage of Rhodamine 101 chloride [2]: (The following protocol is for reference only and should be appropriately adjusted according to your specific experiments.)
1. Preparation of Stock Solution
Dissolve approximately 3-5 mg of Rhodamine 101 powder in 0.3 mL of DMSO, then add distilled water to achieve a final concentration of 1 mg/mL.
Note: The stock solution can be stored stably for at least 3 weeks at 4°C in the dark.
2. Preparation of Working Solution (for combined staining with Mithramycin in ethanol-fixed cells)
Dilute the stock solution with 0.15 M sodium chloride aqueous solution to achieve a Rhodamine 101 working solution concentration of 1.0 μg/mL.
3. Cell Staining
1) Preparation of ethanol-fixed cell samples: Fix the target cells (e.g., CHO cells) in 70% ethanol for at least 18 hours in advance, then centrifuge to remove the ethanol fixative.
2) Resuspend the centrifuged cells in an appropriate amount of the prepared Rhodamine 101-Mithramycin dual-dye working solution.
3) Incubate the cell-dye mixture at room temperature for at least 10 minutes to ensure thorough cell staining.
4. After incubation, analyze the stained cells using flow cytometry.