Rhodamine 101 chloride is a fluorescent dye with excitation/emission wavelengths of 565/595 nm, serving as a reference standard for fluorescence quantum yield measurements.

Usage of Rhodamine 101 chloride [2]: (The following protocol is for reference only and should be appropriately adjusted according to your specific experiments.)
1. Preparation of Stock Solution
Dissolve approximately 3-5 mg of Rhodamine 101 powder in 0.3 mL of DMSO, then add distilled water to achieve a final concentration of 1 mg/mL.
Note: The stock solution can be stored stably for at least 3 weeks at 4°C in the dark.
2. Preparation of Working Solution (for combined staining with Mithramycin in ethanol-fixed cells)
Dilute the stock solution with 0.15 M sodium chloride aqueous solution to achieve a Rhodamine 101 working solution concentration of 1.0 μg/mL.
3. Cell Staining
1) Preparation of ethanol-fixed cell samples: Fix the target cells (e.g., CHO cells) in 70% ethanol for at least 18 hours in advance, then centrifuge to remove the ethanol fixative.
2) Resuspend the centrifuged cells in an appropriate amount of the prepared Rhodamine 101-Mithramycin dual-dye working solution.
3) Incubate the cell-dye mixture at room temperature for at least 10 minutes to ensure thorough cell staining.
4. After incubation, analyze the stained cells using flow cytometry.

The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.

Ethanol: 11 mg/mL (20.87 mM), Sonication is recommended.

H2O: 1.5 mg/mL (2.85 mM), Sonication is recommended.